A kind of method that adopts cellulose-containing raw material to prepare reducing sugar

[0038] This example is used to illustrate the method for preparing reducing sugar from cellulose-containing raw materials provided by the present invention.

[0039] The rice straw was dried at 100° C. so that the water content therein was 1.5% by weight. And the obtained rice stalks were crushed into powders with a particle size less than 212 microns, then mixed evenly with a sodium hydroxide solution with a concentration of 1.0% by weight in a weight ratio of 2:100, and then in a microwave digestion apparatus (purchased from Shanghai Yiyao Instrument Technology Development Co., Ltd., model WX-8000, the same below), microwave digestion was performed at 100°C for 5 minutes. Wherein, the microwave frequency used in the microwave digestion is 2.45GHz, and the pressure used is 0.1MPa. The microstructure of the cellulose-containing raw material obtained after microwave digestion is as follows: figure 2 shown. from figure 2 It can be seen that the surface and internal structure

[0041] This example is used to illustrate the method for preparing reducing sugar from cellulose-containing raw materials provided by the present invention.

[0042] The corn stover was dried at 105° C. so that the water content therein was 1.8% by weight. And the obtained corn stalks were crushed into powder particles with a particle size of less than 270 microns, and then mixed evenly with a potassium hydroxide solution with a concentration of 2% by weight in a weight ratio of 4:100, and then carried out in a microwave digestion apparatus at 150°C. Microwave digestion for 30 minutes. Wherein, the microwave frequency used in the microwave digestion is 2.45GHz, and the pressure used is 0.8MPa. The obtained microwave digestion product was transferred to a hydrolysis vessel, and its pH value was adjusted to 4.8 with hydrochloric acid with a concentration of 5% by weight, and then 3% by weight of cellulase (purchased The hydrolysis was carried out at 40° C. for 96 hours from Japan

[0044] This example is used to illustrate the method for preparing reducing sugar from cellulose-containing raw materials provided by the present invention.

[0045] The wheat straw was dried at 95° C. so that the water content therein was 1.6% by weight. And the obtained wheat stalks were crushed into powder particles with a particle size of less than 500 microns, and then mixed evenly with ammonia water with a concentration of 3% by weight in a weight ratio of 3:100, and then microwave digestion was carried out at 135°C in a microwave digestion apparatus for 10 minute. Wherein, the microwave frequency used in the microwave digestion is 2.45GHz, and the pressure used is 0.3MPa. The obtained microwave digestion product was transferred to a hydrolysis vessel, and its pH value was adjusted to 4.2 with hydrochloric acid with a concentration of 8% by weight, and then 5% by weight of cellulase (purchased The hydrolysis was carried out at 38° C. for 84 hours (from Sigma-Aldrich Compa