Vaccine for preventing and/or treating dengue fever

[0028] Example 1. Application of substances inhibiting mosGCTL-3 gene expression in inhibiting dengue virus replication

[0029] The C-type lectin isoform 3 of Aedes aegypti, also known as mosGCTL-3 protein, is shown in Sequence 1 of the Sequence Listing. The gene encoding the mosGCTL-3 protein is also called the mosGCTL-3 gene, and the coding region of its cDNA is shown in sequence 2 of the sequence listing.

[0030] 1. Design dsRNA

[0031] The dsRNA (dsRNA-1) designed to inhibit the expression of the mosGCTL-3 gene, that is, the double-stranded RNA corresponding to the double-stranded DNA shown in nucleotides 1 to 333 from the 5' end of Sequence 2 of the Sequence Listing.

[0032] A dsRNA (dsRNA-2) designed to inhibit GFP gene expression, the double-stranded RNA shown in SEQ ID NO: 19 of the Sequence Listing.

[0033] 2. Application of substances that inhibit the expression of mosGCTL-3 gene in inhibiting the replication of dengue fever type 2 virus

[0034] 1. Divide Aedes

[0046] Example 2. Application of substances inhibiting mosGCTL-26 gene expression in inhibiting dengue virus replication

[0047] The C-type lectin isoform 26 of Aedes aegypti is also known as mosGCTL-26 protein, as shown in sequence 3 of the sequence listing. The gene encoding the mosGCTL-26 protein is also called the mosGCTL-26 gene, and the coding region of its cDNA is shown in sequence 4 of the sequence listing.

[0048] 1. Design dsRNA

[0049] The dsRNA (dsRNA-3) designed to inhibit the expression of the mosGCTL-26 gene, that is, the double-stranded RNA corresponding to the double-stranded DNA shown in nucleotides 31-381 from the 5' end of sequence 4 of the sequence listing.

[0050] 2. Application of substances inhibiting the expression of mosGCTL-26 gene in inhibiting the replication of dengue fever type 2 virus

[0051] 1. Divide Aedes aegypti into two groups and deal with them as follows:

[0052] Experimental group: each Aedes aegypti mosquito was injected with 2 mi

[0063] Example 3. Application of substances inhibiting mosGCTL-15 gene expression in inhibiting dengue virus replication

[0064] The C-type lectin subtype 15 of Aedes aegypti is also called mosGCTL-15 protein, as shown in SEQ ID NO: 5 of the sequence listing. The gene encoding the mosGCTL-15 protein is also called the mosGCTL-15 gene, and the coding region of its cDNA is shown in Sequence 6 of the Sequence Listing.

[0065] 1. Design dsRNA

[0066] The dsRNA (dsRNA-4) designed to inhibit the expression of the mosGCTL-15 gene, that is, the double-stranded RNA corresponding to the double-stranded DNA shown in nucleotides 79 to 407 from the 5' end of sequence 6 of the sequence listing.

[0067] 2. Application of substances that inhibit the expression of mosGCTL-15 gene in inhibiting the replication of dengue fever type 2 virus

[0068] 1. Divide Aedes aegypti into two groups and deal with them as follows:

[0069] Experimental group: each Aedes aegypti mosquito was injected with 2