Hybridoma cell strain secreting anti-maize chlorotic dwarf virus monoclonal antibody, secreted antibody, application and kit prepared from monoclonal antibody

A hybridoma cell line and dwarf virus technology, which is applied in antiviral immunoglobulin, analytical materials, and microbial-based methods, can solve the problems of complex operation, high cost, and high professional skill requirements for operators

Inactive Publication Date: 2015-02-04
QINGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU P R CHINA
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for high accuracy and sensitivity testing on crops like rice or wheat without requiring costly equipment. It also enables effective monitoring of diseases caused by this disease through its presence.

Problems solved by technology

This patented describes how certain types of insects called Chikungunya fever vims affect crops such as rice crop production worldwide. They cause damage through feeding onto these crops during growth periods when they become sick due to exposure to this type of virus. To protect against them, we developed an assay method based upon molecular techniques like Western Blotting Disease Vaccine(WBVD), which uses DNA extracted from bacteria grown inside tumors known commonly associated with CHIKKARCHVERM VIZAKI syndrome. However, while effective in detecting other forms of alkenema witch haemorrhagulants, WEBVM does not work well enough because many nonhost species may contaminate the sample being tested.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hybridoma cell strain secreting anti-maize chlorotic dwarf virus monoclonal antibody, secreted antibody, application and kit prepared from monoclonal antibody
  • Hybridoma cell strain secreting anti-maize chlorotic dwarf virus monoclonal antibody, secreted antibody, application and kit prepared from monoclonal antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] The hybridoma cell line MCDV secreting anti-maize chlorotic dwarf virus monoclonal antibody was preserved in the China Center for Type Culture Collection (CCTCC) (Wuchang Luo, Wuhan City, Hubei Province) on April 26, 2014. Jiashan, Wuhan University, Collection Center), the collection number is CCTCC No: C201486, it can secrete monoclonal antibody against maize chlorotic dwarf virus.

[0013] Anti-maize chlorotic dwarf virus monoclonal antibody ascitic fluid indirect ELISA titer reached 10 -7 , the antibody type and subclass are IgG 1 , kappa chain, the monoclonal antibody can have specific immunological binding reaction with the coat protein subunit of maize chlorotic dwarf virus.

[0014] Monoclonal antibodies against maize chlorotic dwarf virus have specific immune reactions only with maize chlorotic dwarf virus, but with rice tungro spherical virus (RTSV), maize dwarf mosaic virus (Maize dwarf mosaic Virus (MDMV), Maize rough dwarf Virus (MRDV), Maize chlorotic mottle

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a hybridoma cell strain secreting an anti-maize chlorotic dwarf virus (MCDV) monoclonal antibody. The hybridoma cell strain is collected in China Center for Type Culture Collection with the collection name of hybridoma cell MCDV and the collection number of CCTCC NO: C201486. The hybridoma cell strain provided by the invention has the beneficial effects that, 1) the provided hybridoma cell strain secretes the anti-maize chlorotic dwarf virus specific monoclonal antibody, TAS (three antibodies sandwich)-ELISA (enzyme linked immunosorbent assay) and dot-ELISA and other immunological methods established by taking the monoclonal antibody as the core and kits established by using the methods can detect maize chlorotic dwarf virus with high specificity, accuracy and sensitivity; 2) by using the monoclonal antibody prepared according to the invention to detect the maize chlorotic dwarf virus, expensive instruments and reagents are not required; and 3) the monoclonal antibody prepared according to the invention can be effectively used for detecting the maize chlorotic dwarf virus in imported corn and other maize chlorotic dwarf virus hosts.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner QINGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU P R CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap