Microimaging optical system of imaging flow cytometry

The invention discloses a microimaging optical system of an imaging flow cytometry and relates to the field of optical instruments applied to biology and medicine. The system solves the problems of an existing optical system of the imaging flow cytometry that the number of spectral imaging channels is small, imaging quality can not reach diffraction limit, and the resolution of the cytometry can not be improved. The system comprises a microobjective, a secondary imaging lens set, a multi-spectral light splitter, a multi-spectral imaging lens and a TDI camera. Full-wave band high-resolution amplification imaging of a sample to be tested is achieved through the achromatic microobjective, then an intermediate image plane is obtained through the secondary imaging lens set, a field stop is placed at the position of the intermediate image plane, collision is conducted through the secondary imaging lens set, stack splitting is conducted through the multi-spectral light splitter, and then light is converged on the TDI camera through the multi-spectral imaging lens. According to the system, full-spectrum subband multiple structures are adopted, it is guaranteed that the imaging quality of the imaging optical system can reach diffraction limit in any spectrum channel, and the minimum detection size of the whole system reaches 40 nm.