Immunity magnetic bead test strip for testing tumor marker CA72-4 and preparing method thereof

A technology of tumor markers and immune magnetic beads, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that there is no report on the detection of tumor marker CA72-4, it is not suitable for early screening of cancer patients, and the operation steps are complicated, etc. problem, to achieve the effect of improving sensitivity and accuracy, high sensitivity and specificity, and high reliability

Inactive Publication Date: 2015-09-23
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0008] 1. At present, the clinical methods for detecting CA72-4 are mainly traditional immunological detection methods, such as electrochemiluminescence, ELISA, and enzyme-linked fluorescent immunoassay system. The operation process requires special laboratories and professionals for detection and operation. The

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  • Immunity magnetic bead test strip for testing tumor marker CA72-4 and preparing method thereof

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[0040] Example 1: Preparation and application of an immunomagnetic bead test strip for detecting tumor marker CA72-4

[0041] Step 1: Preparation of Nano Magnetic Bead Probe

[0042] 1) Activation: Take 2 morpholinoethanesulfonic acid (MES) solution at pH 5.50.01M as activation buffer, take 4mg / ml liquid carboxyl magnetic beads (belonging to carboxyl nanomagnetic beads, the particle size of the magnetic beads is about 10nm) 500ul In a 2ml centrifuge tube, sonicate for about 30min, centrifuge with a refrigerated centrifuge (18℃, 13000rpm, 5min), discard the supernatant, take 500ul activation buffer to resuspend the magnetic beads on the wall, and mix well on the vortex shaker (e.g. It can be sonicated for several minutes if necessary), continue centrifugation, the parameters are the same. After washing twice with activation buffer, resuspend the magnetic beads in 500ul activation buffer. Weigh 4 mg of (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (abbreviated EDC) an

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Abstract

The invention provides an immunity magnetic bead test strip for testing tumor marker CA72-4 and a preparing method thereof. The immunity magnetic bead test strip comprises a sample pad, a connecting pad, a nitrocellulose film, a water absorbing pad and a backing board. The sample pad, the connecting pad, the nitrocellulose film and the water absorbing pad are stacked in order on the backing board. The stacked parts along the length of the backing board are 0.5 to 2 mm in length. An immunochromatographic method for use is a double-antibody sandwich method; two antibodies are an anti-CA72-4 monoclonal antibody and anti-CA72-4 monoclonal antibody B72.3. The preparing method mainly relates to the preparation of nano magnetic bead probes. After the immunochromatography with the strip, a magnetic intensity tester is used to analyze and acquire CA72-4 quantitative results; the preparing method is simple and fast and is higher in sensitivity and specificity.

Description

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Claims

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Application Information

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Owner SHANGHAI JIAO TONG UNIV
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