The invention relates to a splitting
reagent for removing non-nuclear red blood cells in a biological sample and an application of the
reagent to
DNA extraction. The splitting
reagent for removing non-nuclear red blood cells comprises a
metal ion chelating agent,
inorganic salts, a surfactant and a solution buffer
system. The invention further provides an application method of the splitting reagent to
DNA extraction. The splitting reagent for removing non-nuclear red blood cells provided by the invention overcomes defects that a conventional product is poor in stability, needs frozen preservation and needs to be preheated for use after being taken out, the red blood cells in blood samples or
tissue cell samples of people, mice and the like can be effectively removed through splitting, andnucleated cells in the samples are separated and enriched. The splitting reagent is used for extracting
nucleic acid(
DNA) of the nucleated cells, and has the advantages of being stable in components,safe, environmental-friendly, high in splitting
efficacy of the red blood cells, and quick and simple to operate. The reagent can be stored at normal temperature for a long term,
cold chain transportation is not needed, and the preparation and use cost is low.