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43results about How to "Simple and efficient operation" patented technology

Integrated ion sensor

The invention provides an integrated ion sensor which is characterized in that a working electrode assembly and a reference electrode assembly are arranged on the surface of a substrate, wherein, theworking electrode assembly comprises an ion-selective electrode (ISE) arranged on the surface of the substrate, a lead electrically connected with the ISE, and a blocking layer covering part of the ISE and lead; and the reference electrode assembly comprises a reference electrode arranged on the surface of the substrate, a lead electrically connected with the reference electrode, a blocking layercovering part of the lead, and an enclosing body arranged on the surface of the substrate, wherein, the enclosing body is provided with an exchanging interface for liquids to flow into the enclosing body and filled with electrolytes. By arranging the reference electrode and the working electrode on the same substrate at the same time, the integrated ion sensor becomes more user-friendly.
Owner:MIDDLELAND SENSING TECH

In vitro detection kit for TAFI content

The invention discloses an in vitro detection kit for thrombin-activatable fibrinolysis inhibitor (TAFI) content. The kit is used for the in vitro detection of the TAFI content by using the biological property that a polyamidoamine (PAMAM) dendritic polymer can be combined with an antibody and combining the traditional enzyme-linked immuno sorbent assay (ELISA) method, and the kit develops a new in vitro diagnostics method for the TAFI and improves the sensitivity of the TAFI in vitro detection. The kit comprises an antibody coated ELISA plate, a sample diluent, a TAFI standard, a detergent, a PAMAM-labeled horse radish peroxidase-linked antibody, an antibody diluent, color development buffer solution, 30 percent hydrogen peroxide, o-phenylenediamine and stop solution. The kit overcomes the defects of expensive equipment, long detection time and the like in the prior art; compared with other detection kits, the kit is simple, convenient and fast in operation, low in cost, sensitive in diagnosis and has low detection limit and the like; the kit for auxiliary diagnosis improves the detection rate and the accuracy of related diseases, and fulfills the purposes of early discovery and early treatment; and the kit reduces unnecessary pain and medicinal expense of examined persons, and improves the quality of life of the examined persons.
Owner:LIAONING MEDI BIOTECH CO LTD

Comprehensive utilization process for extracting calcium ions from South China Sea calcareous sands and treating algae sewage, and application thereof

PendingCN113666407ASimple and efficient operationUniform reinforcement strength distributionBiological water/sewage treatmentCalcium/strontium/barium chloridesBiocement DCalcium carbonate
The invention discloses a comprehensive utilization process for extracting calcium ions from South China Sea calcareous sands and treating algae sewage. The comprehensive utilization process comprises the following steps: weighing the South China Sea calcareous sands, feeding into a stone crusher P-5, and mechanically crushing to form small-particle-size particles; feeding diluted hydrochloric acid and the crushed South China Sea calcareous sands into a calcium ion extraction reactor P-1 to react to generate a calcium chloride solution, CO2 gas and water; carrying out primary separation on a reaction mixture through a sedimentation tank P-2, refluxing unreacted South China Sea calcareous sands to P-1, carrying out secondary reaction to generate a calcium chloride solution, and collecting to obtain a final extraction product calcium chloride solution; feeding the remaining mixture into a microfiltration system P-3, separating and purifying, and reacting a product solution in an acid-base neutralization reactor P-6; and introducing CO2 gas into a runway type algae reactor P-4, and purifying domestic sewage through autotrophy and heterotrophy growth. The calcium carbonate capable of being used for biological cement reinforcement is higher in crystal form strength and more uniform in distribution; and zero emission, economy and environmental protection are realized.
Owner:SUN YAT SEN UNIV

Reagent for removing non-nuclear red blood cells in biological sample and application of reagent to DNA extraction

PendingCN109852607AIncrease concentrationEasy to useMicrobiological testing/measurementDNA preparationIonChemistry
The invention relates to a splitting reagent for removing non-nuclear red blood cells in a biological sample and an application of the reagent to DNA extraction. The splitting reagent for removing non-nuclear red blood cells comprises a metal ion chelating agent, inorganic salts, a surfactant and a solution buffer system. The invention further provides an application method of the splitting reagent to DNA extraction. The splitting reagent for removing non-nuclear red blood cells provided by the invention overcomes defects that a conventional product is poor in stability, needs frozen preservation and needs to be preheated for use after being taken out, the red blood cells in blood samples or tissue cell samples of people, mice and the like can be effectively removed through splitting, andnucleated cells in the samples are separated and enriched. The splitting reagent is used for extracting nucleic acid(DNA) of the nucleated cells, and has the advantages of being stable in components,safe, environmental-friendly, high in splitting efficacy of the red blood cells, and quick and simple to operate. The reagent can be stored at normal temperature for a long term, cold chain transportation is not needed, and the preparation and use cost is low.
Owner:SHANGHAI XINGYAO MED TECH DEV CO LTD

Five-prevention interlocking mechanism

The invention relates to a five-prevention interlocking mechanism, which comprises a lower door ground interlocking unit and a load isolation and ground isolation interlocking unit. The lower door ground interlocking unit consists of a door hanging arm plate, a lower door interlocking connection pull rod, an interlocking connector, a crank arm and a lower door interlocking shaft shaft, wherein the door hanging arm plate is connected with the lower end of the lower door interlocking connection pull rod, the upper end of the lower door interlocking connection pull rod is connected with the interlocking connector, the interlocking connector is connected with one end of the crank arm and the other end of the crank arm is connected with the lower door interlocking half shaft. A ground baffle which is arranged in the front of a ground operating shaft is locked or unlocked under the situation that a lower door is not hung or hung on the door hanging arm plate to control the opening/closing of a ground switch and the opened/locked state of the lower door. The load isolation and ground isolation interlocking unit consists of an isolation indicating wheel, a ground indicating wheel, a cam and a camshaft. After an isolating switch and the ground switch are closed, the ground baffle and an isolating baffle are respectively locked in the front of the ground operating shaft and an isolation operating shaft to prevent the ground switch and the isolating switch from being mistakenly operated. The five-prevention interlocking mechanism has the advantages of small size, convenience in installation, simplicity and convenience in operation and high safety and reliability.
Owner:SHENYANG HAOCHENG ELECTRICAL SCI & TECH

Method and special kit for detection of CNV label of cattle VAMP7 gene

ActiveCN111172295AThe method is accurate and reliableSimple and efficient operationMicrobiological testing/measurementAnimal scienceGenetic resources
The invention discloses a method and special kit for detection of a CNV (copy number variations) label of a cattle VAMP7 gene. The method comprises the steps: adopting the whole genome DNA of cattle as a template, performing amplification on a partial fragment of the CNV region of the cattle VAMP7 gene and a partial fragment of a reference gene BTF3 separately through a real-time fluorescence quantitative PCR, and dividing the quantitative results into an insertion type, a deletion type and a normal type based on 2 * 2<-triangle triangle Ct> so as to identify the type of the copy number variations (CNV) of the cattle VAMP7 gene. It is shown through correlation analysis of production data of Yunling cattle, Xianan cattle, Pinan cattle and Jiaxian red cattle showed that the height at hip cross of individuals of the normal type is significantly higher than that of individuals of the insertion type and deletion type. Through detection of the CNV label closely related to the growth traits of Yunling cattle at the DNA level, the method can be applied to quick establishment of dominant populations of genetic resources of Yunling cattle and other local cattle varieties, molecular marker-assisted selective breeding of cattle is quickened, and the method is simple and fast, and has easy promotion and application.
Owner:NORTHWEST A & F UNIV

Cloud technology-based voice recognition and intelligent black broadcast identification method

ActiveCN109995450AImprove recognition efficiency and recognition accuracySimple and efficient operationBroadcast observation/testing/troubleshootingCharacter and pattern recognitionAudio signalBroadcast radiation
The invention discloses a cloud technology-based voice recognition and intelligent black broadcast identification method, which comprises the following implementation steps: 1, demodulating a signal which is artificially identified as black broadcast or a previously stored signal, and sending the demodulated signal to a cloud for voice recognition to obtain a large number of black broadcast audio-to-text data samples; 2, carrying out unsupervised learning and training on the known black broadcast character data sample by adopting DNN, and extracting black broadcast characteristic keywords to form a keyword library; 3, sending the demodulated broadcast audio signal to a cloud for speech recognition to obtain broadcast audio-to-text content; and 4, carrying out similarity matching on the text content and the keyword library to obtain a similarity matching result. The broadcast signal is subjected to voice recognition through the cloud technology, operation is easy and convenient, and extra cost is not needed; and the DNN is adopted to carry out unsupervised learning and training on the black broadcast characteristic keywords, so that the recognition efficiency and the recognition accuracy are improved.
Owner:NANJING UNIV OF AERONAUTICS & ASTRONAUTICS

Method for maintaining bearings of crane span structures of cutter suction dredgers

The invention discloses a method for maintaining bearings of crane span structures of cutter suction dredgers. The method comprises the following steps: mounting a bearing maintaining device (4) on anoriginal reamer crane span structure (3), wherein the bearing maintaining device (4) has a cylindrical structure, can support the reamer crane span structure (3) and can serve as a rolling shaft forlongitudinal rolling of the reamer crane span structure (3), the mounting position of the bearing maintaining device (4) is close to a bearing (5), and the bearing maintaining device (4) can stretch out or retract in relative to the reamer crane span structure (3); and calculating the weight of the reamer crane span structure (3), determining the mounting position of the bearing maintaining device(4), calculating the size of the bearing maintaining device (4), mounting a stopping block (9) on a main hull deck (8) as an accurate limiter for the longitudinal rolling distance of the reamer cranespan structure (3) so as to ensure that the bearing (5) can smoothly slip out from a bearing block upwards obliquely at an angle of 30 degrees and to maintain or change the bearing (5). The method for maintaining bearings of crane span structures of cutter suction dredgers has the beneficial effects that when required to be repaired or changed, the bearing does not need to be dragged to a repairsite with the aid of external force or a floating crane, but the bearing can be repaired or changed effectively and rapidly at low cost.
Owner:瑞港(天津)工程技术有限公司

Method for breeding, culturing and preserving paratylenchus by using carrot callus

The invention discloses a method for breeding, culturing and preserving paratylenchus by using carrot callus, belonging to the field of plant-parasitic nematode culture. The method comprises the following steps of: (1) under a sterile condition, soaking carrots with alcohol, scorching, peeling and slicing the carrots, putting carrot slices into a sterilized culture dish, sealing an opening of the sterilized culture dish, putting into a culture box, and culturing till the carrot slices grow callus; (2) sterilizing the paratylenchus with a streptomycin sulfate solution, centrifuging and collecting, eluting with sterile water, centrifuging and collecting again, preparing a paratylenchus suspension with sterile water, and dripping the suspension on the carrotcallus; and (3) putting the carrot callus inoculated with the paratylenchus into the culture box, and culturing. In the method, a culture medium is not required for culturing, so that the operation is simple and convenient; and sterilization of the streptomycin sulfate solution does not affect the activity of the paratylenchus, so that the aim of breading the paratylenchus simply, efficiently and largely is achieved, the paratylenchus can be preserved for a long time, and parasitism and pathogenicity of the paratylenchus on a plant are kept.
Owner:SOUTH CHINA AGRI UNIV

Extraction method for high-purity and high-extraction-rate pyrethrin

InactiveCN106349075AEquipment generalSimple and efficient operationCarboxylic acid esters separation/purificationEthyl acetateEthyl ester
The invention provides an extraction method for high-purity and high-extraction-rate pyrethrin. The extraction method comprises the following steps: (1) drying pyrethrum petals at the temperature of 100 to 110 DEG C for 20 to 30 minutes, smashing the petals, and screening the smashed petals with a screen of 80 to 100 meshes to obtain pyrethrum powder; (2) placing the pyrethrum powder, cellulase and a mixed solvent into a soxhlet extractor, heating the soxhlet extractor until reflux occurs, irradiating for 40 to 60 minutes with ultraviolet rays, continuously refluxing for 4 to 6 hours, and taking an extracting solution for later use, wherein the mixed solvent is prepared from ethyl acetate and normal octane according to a mass ratio of 1 to (0.5 to 0.8); (3) distilling the extracting solution, respectively collecting fractions at the temperature of 75 to 80 DEG C and the temperature of 122 to 130 DEG C, and collecting remnants which are the required pyrethrin. By the adoption of the ultraviolet rays assisting in extraction to extract the pyrethrin, the extraction method has the advantages of high efficiency, conventional equipment, simplicity and convenience for operation, high product purity and high extraction rate, and is suitable for large-scale production.
Owner:安吉威龙塑木环保材料有限公司

Cord strapping method

ActiveCN102120500ASimple and efficient operationSimple structureBundling machine detailsHollow cylinderKnot tying
The invention discloses a cord strapping and knot tying method, which comprises the following steps: pre-winding multiple cord knots on a support in the shape of a hollow cylinder or other shapes by various required strapping methods and knot tying methods, and placing the cord knots on the object parts needing to be strapped. The free end of the cord is wound around an object needing to be strapped and pulled back by a nipper or other devices passing through the inside of the support so that the free end of the cord and the nipper pass through the centers of multiple cord knots pre-wound on the surface of the support in the shape of a hollow cylinder or other shapes; a pair of pushing tongs or a pushing rod pushes the multiple cord knots pre-wound on the support one by one along the knot tying direction of the cord knots so that the cord knots are wound and knotted with the free end of the cord wound around the strapped object; and the core knots are pushed to the object needing to be strapped and firmly tied to realize an effective strapping and knot tying effect.
Owner:苏红 +1

Preparation method of environment-friendly economic luminescent carbon quantum dot

InactiveCN103303902AThe preparation process equipment is simpleSimple and efficient operationMaterial nanotechnologyNano-carbonMuffle furnaceChemistry
The invention discloses a preparation method and application of an environment-friendly economic luminescent carbon quantum dot. The method disclosed by the invention mainly comprises the following steps of: with bagasse as a carbon source material, adding the bagasse into a mineralizer aqueous solution, and heating by using a hydro-thermal synthesis reaction kettle in a muffle furnace at the temperature of 180 DEG C for 4h; centrifuging a brownish black reaction liquid at a high speed, and then, collecting a supernatant for dialyzing; collecting the reaction liquid in a dialysis bag, cooling the reaction liquid, and then, transferring the reaction liquid into a freezer drier for freeze-drying to finally obtain a powdery carbon quantum dot. The preparation method disclosed by the invention has the characteristics of simplicity, convenience and rapidness in operation as well as simplicity in preparation process and equipment; and the environment-friendly economic luminescent carbon quantum dot is made of daily wastes as raw materials and is low in cost, environment-friendly and easy to popularize. The carbon quantum dot prepared by the invention has the excellent characteristics of favorable dispersibility, water solubility, fluorescent stability, biocompatibility and the like, and is successfully applied to living cell imaging and marking.
Owner:JIANGSU UNIV

Methods for sampling and detecting microorganisms on contact surface

InactiveCN105087753ASimple and fast operationImprove sampling effectMicrobiological testing/measurementWettingFilter paper
The invention discloses methods for sampling and detecting microorganisms on a contact surface and belongs to the technical field of microbiological detection. The sampling method comprises the following steps: 1), sterile filter paper is placed in a sterile wetting liquid containing peptone, a surfactant and sodium chloride for wetting; 2), a to-be-detected surface is covered with the wetted filter paper, and the filter paper is retained for sampling. The detection method further comprises the following steps: the filter paper after sampling is arranged in a phosphate buffer, uniform mixing, sampling and counting are performed, and the quantity of microorganisms on unit detected surface is obtained through calculation. The sampling method is simple to operate and high in sampling efficiency, causes little mechanical damage to the microorganisms on the contact surface, is particularly suitable for batch sampling of the microorganisms on table board type contact surfaces and can improve the sampling efficiency substantially. Meanwhile, the detection precision and accuracy of the detection method are high, and the detection efficiency can be improved substantially.
Owner:HENAN AGRICULTURAL UNIVERSITY
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