Tyrosinase fluorescent biosensor and atrazine detection method thereof

A biosensor and tyrosinase technology, applied in the field of tyrosinase fluorescent biosensor and its detection of atrazine, can solve the problems of groundwater and environmental water pollution, long half-life, time and high cost, and achieve low cost , the effect of easy operation

Pending Publication Date: 2019-05-21
CHINA AGRI UNIV
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Problems solved by technology

However, atrazine also has certain disadvantages: its long half-life in the soil may cause phytotoxicity to subsequent crops if used improperly; in addition, the long half-life in the soil also increases the environmental risk effect of atrazine. Under the action of the leaching effect, it may cause pollution to groundwater and envi

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[0020] The invention provides a preparation and application method of a tyrosinase fluorescent biosensor for detecting atrazine. The present invention will be further described below in conjunction with specific embodiments.

[0021] 1. Instruments and reagents:

[0022] Varian Cary Eclipse Fluorescence Spectrophotometer.

[0023] Citric acid, ammonia, dopamine, tyrosinase and atrazine are all purchased from Aladdin. When used, except tyrosinase is prepared with phosphate buffer, the others are diluted with ultrapure water to a working solution of the required concentration.

[0024] 2. Design and detection mechanism of tyrosinase fluorescent biosensor

[0025] The biosensor is an analysis system composed of biometric components and physical and chemical signal sensors. Biometric components are substances that can specifically interact with a certain compound, such as enzymes. The changes produced by this specific interaction are converted into a certain signal by a physical and chemica

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Abstract

The invention discloses a tyrosinase fluorescent biosensor and an atrazine detection method thereof. The tyrosinase fluorescent biosensor is composed of nitrogen-doped graphene quantum dots, tyrosinase and dopamine, wherein the nitrogen-doped graphene quantum dot is used as a fluorescent probe; and the dopamine is used as a catalytic substrate. The tyrosinase fluorescent biosensor is adopted to becombined to a fluorescence spectrophotometer for rapid and sensitive quantitative analysis on atrazine. The method combines the tyrosinase fluorescent biosensor with fluorescence spectrometry for thefirst time for atrazine detection. Compared with the traditional chromatographic analysis method, the method disclosed in the ivnention uses a fluorescent spectrometer with relatively low cost, relatively simple operation and miniaturization potential for rapid and sensitive quantitative detection on atrazine.

Description

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Claims

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Application Information

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Owner CHINA AGRI UNIV
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