Preparation method for konjac mannatide
A technology of konjac mannan and glycopeptides, which is applied in the field of preparation of glycopeptides and konjac mannan peptides, can solve problems such as unseen mannan peptides, and achieve the effect of broadening sources and broadening preparation methods
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[0030] Example 1
[0031] 1) Fermentation: 1g of konjac fly powder is added to 100mL fermentation medium, and the symbiotic fermentation is carried out with thermostable proteolytic Bacillus and Bacillus pumilus. After the fermentation is completed, the fermentation broth is centrifuged at 8000r / min for 30min at 4°C to remove the bacteria. The supernatant is added with 2 times purified water, the pH is adjusted to 3.8 with trichloroacetic acid, and the precipitation is removed after standing. The supernatant is the konjac polypeptide liquid.
[0032] Among them, the composition of the medium is: peptone 1g, beef extract 1.5g, NaNO 3 0.2g, K 2 HPO 4 0.2g, KCl0.1g, MgSO 4 ·7H 2 O0.1g, FeSO 4 0.005g, 100mL of distilled water. The pH of the culture medium is 7, the ratio of heat-resistant proteolytic Bacillus to Bacillus pumilus is 1:1, and the concentration of the bacterial solution is diluted to 5×10 7 A / mL, the inoculum volume is 12% (V / V, the inoculum volume refers to the volum
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[0036] Example 2
[0037] 1) Fermentation: Add 3g of konjac fly powder to 100mL fermentation medium, and use thermotolerant proteolytic Bacillus and Bacillus pumilus for symbiotic fermentation. After the fermentation is completed, the fermentation broth is centrifuged at 9000r / min for 15min at 4℃ to remove the bacteria, the supernatant is added with 3 times purified water, the pH is adjusted to 3.9 with trichloroacetic acid, and the precipitation is removed after standing. The supernatant is the konjac polypeptide liquid.
[0038] Among them, the composition of the medium is: peptone 1g, beef extract 1.5g, NaNO 3 0.08g, K 2 HPO 4 0.25g, KCl0.03g, MgSO 4 ·7H 2 O0.08g, FeSO 4 0.01g, 100mL of distilled water. The pH of the medium is 8, the ratio of heat-resistant proteolytic Bacillus to Bacillus pumilus is 1:2, and the concentration of the bacterial solution is diluted to 8×10 7 Pieces / mL, the inoculum amount is 10% (V / V), the fermentation temperature is 32°C, and the fermentation
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[0042] Example 3
[0043] 1) Fermentation: 4g of konjac fly powder was added to 100mL fermentation medium, and thermotolerant proteolytic Bacillus and Bacillus pumilus were used for symbiotic fermentation. After the fermentation is completed, the fermentation broth is centrifuged at 10000r / min for 30min at 4℃ to remove the bacteria. The supernatant is added with 4 times purified water, the pH is adjusted to 4 with trichloroacetic acid, and the precipitation is removed after standing. The supernatant is the konjac polypeptide liquid.
[0044] Among them, the composition of the medium is: peptone 1g, beef extract 1.5g, NaNO 3 0.39g, K 2 HPO 4 0.04g, KCl0.05g, MgSO 4 ·7H 2 O0.05g, FeSO 4 0.001g, 100mL distilled water. The pH of the culture medium is 8.5, the ratio of heat-resistant proteolytic Bacillus to Bacillus pumilus is 1:3, and the concentration of the bacterial solution is diluted to 7×10 8 Pieces / mL, the inoculum amount is 8% (V / V), the fermentation temperature is 34°C, an
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