Primers for detecting bovine ephemeral fever virus and detection kit

In 2005, Israeli researchers Stram et al. established TaqMan-MGB fluorescent quantitative RT-PCR for BEFV antigen gene G to detect BEFV detection, which can effectively detect the local isolate Yaqum-00 and the Australian reference strain BB7721, but due to There are large variations in the G gene of different strains, and it is difficult to ensure the absolute matching between the probe and the Chinese BEFV strain. Chinese invention patent application 201210219927.5 discloses the TaqMan-MGB fluorescence quantitative RT-PCR method for the Chinese BEFV strain. In order to solve the matching problem, two degenerate bases are introduced into the probe. This patent application is based on the ordinary probe. The MGB modified probe has stronger hybridization ability and is more stable, but it needs to be prepared by a professional company. Increased testing costs