Preparation and application of probe liquid chip for hypersensitive detection of miRNA

A liquid chip and probe technology, applied in the field of miRNA detection, can solve the problems of inability to realize bedside rapid diagnosis, cumbersome and complicated, and costly, etc., and achieve the effect of simple and fast result judgment, high detection sensitivity, and reduced measurement cost

Inactive Publication Date: 2018-06-01
清水湾生物材料(深圳)有限公司
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Benefits of technology

This patented technology allows for faster and more accurate analysis of small amounts (<10 grams) of biological materials such as microorganisms that contain DNA fragments called ribonucleotides. It involves detecting changes caused by these tiny substances during their growth processes. By attaching an oligo nucleic acid tag onto this metal nanoparticle, which helps identify them from other similar particles containing different types of nucleosids. These tags help researchers analyze gene expression patterns related to diseases like cancer cells better than traditional methods.

Problems solved by technology

This patents discuss how tiny DNA called microribons play a crucial role in regulating cellular functions such as growth control by adjusting their levels through specific proteins found within them. They were discovered about 30 years ago when they was isolated during early stages of life. Overall, this discovery provides new ways to study these structures more efficiently than previously possible due to its simplicity and ease of use.

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Embodiment Construction

[0027] In order to facilitate those skilled in the art to better understand the technical solutions of the present invention, the present invention will now be further described in conjunction with specific embodiments.

[0028] Preparation of probes for rapid and ultrasensitive detection of miRNA,

[0029]S1, LNA probe sequence modification: Taking the detection target sequence MiR141 (a tumor marker, the specific sequence is 5'-rUrArArCrArCrUrGrUrCrUrGrGrGrUrArArArArGrArUrGrG-3') as an example, the designed miRNA capture probe sequence is 5'-AT+CTT+ TACC+AGACA+GT+GTTA-3'-CH 2 CH 2 CH 2 -S-S-CH 2 CH 2 CH 2 -OH, the base with "+" is introduced into the chain nucleic acid LNA. The chain nucleic acid LNA means that the 2'-O and 4'-C positions of the β-D-ribofuranose on its corresponding base form a rigid cage structure through shrinkage, and the 3' end of the chain nucleic acid LNA is modified After the disulfide bond is formed, the LNA probe sequence solution containing dis

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Abstract

The invention discloses preparation and application of a probe liquid chip for hypersensitive detection of miRNA. The basic principle of the probe liquid chip is an optical biosensor based on local surface plasma resonance effect of metal nano-arrays. A probe for rapid and hypersensitive detection of miRNA is prepared by the steps as follows: S1, modification of an LNA probe sequence; S2, arrangement of the metal nano-arrays; S3, disulfide reduction of the LNA probe sequence; S4, anchoring of the LNA probe. According to the preparation and application of the probe for rapid and hypersensitivedetection of miRNA, the probe ha high detection sensitivity, the chip is recyclable, and the service life is long.

Description

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Claims

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Application Information

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Owner 清水湾生物材料(深圳)有限公司
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