Tissue culture propagation method of Polygonatum multiflorum
A Polygonatum multiflora, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of increased tissue culture cycle and cost, weak meristematic ability, easy pollution, etc., and achieve easy sterilization Effects of treatment, promotion of multiplication, and non-pollution
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[0043] Example 1
[0044] 1. Preparation of seed embryo: Collect the mature fruits of Polygonatum multiflorum in mid-September, pile them for 1 week, wash the flesh with clean water after the skin is slightly rotten. The seeds were soaked in clean water for 24 hours, during which the water was changed 4 times. Rinse the seeds with running water for 5 min, then use 0.1% HgCl 2 Soak for 5-8min, rinse with sterile water, absorb the moisture on the surface of the seeds with sterile filter paper, cut the treated seeds with dissecting scissors, and take out the seed embryos.
[0045] 2. Sterilization of seed embryos: use 0.1% HgCl 2 Soak the embryos for 8 minutes, and finally rinse with sterile water for 8 times.
[0046] 3. Seed embryo inoculation and callus induction: The sterilized seed embryos are inoculated on the prepared medium. The formula of the medium is: MS+6-BA2.0mg / L+NAA0.2mg / L, per bottle 4 to 5 inoculations. Place it on a non-illuminated culture rack with a temperature o
Example Embodiment
[0051] Example 2
[0052] 1. Preparation of seed embryo: Collect the mature fruits of Polygonatum multiflorum in early October, pile them for 1 week, wash the flesh with clean water after the skin is slightly rotten. The seeds were soaked in clean water for 24 hours, during which the water was changed 4 times. Rinse the seeds with running water for 5 min, then use 0.1% HgCl 2 Soak for 8 minutes, rinse with sterile water, absorb the moisture on the surface of the seeds with sterile filter paper, cut the treated seeds with dissecting scissors, and take out the seed embryos.
[0053] 2. Sterilization of seed embryos: use 0.1% HgCl 2 Soak the embryos for 8 minutes, and finally rinse with sterile water for 8 times.
[0054] 3. Seed embryo inoculation and callus induction: The sterilized seed embryos are inoculated on the prepared medium. The formula of the medium is: MS+6-BA 1.5mg / L+NAA0.15mg / L, per bottle 4 to 5 inoculations. Place it on a non-light culture rack with a temperature of
Example Embodiment
[0058] Example 3
[0059] 1. Preparing seed embryos: Collect the mature fruits of Polygonatum multiflorum in late September, pile them for 1 week, and wash the pulp with water after the skin is slightly rotten. The seeds were soaked in clean water for 24 hours, during which the water was changed 4 times. Rinse the seeds with running water for 5 min, then use 0.1% HgCl 2 Soak for 5-8min, rinse with sterile water, absorb the moisture on the surface of the seeds with sterile filter paper, cut the treated seeds with dissecting scissors, and take out the seed embryos.
[0060] 2. Sterilization of seed embryos: use 0.1% HgCl 2 Soak the seed embryos for 6-7 minutes, and finally rinse with sterile water 8 times.
[0061] 3. Seed embryo inoculation and callus induction: The sterilized seed embryos are inoculated on the prepared medium. The formula of the medium is: MS+6-BA 2.2mg / L+NAA 0.2mg / L, per bottle 4 to 5 inoculations. Place it on a non-illuminated culture rack with a temperature of
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