Pretreatment method of microchemical analysis of pectoral fin strip of Chinese sturgeon

[0030] A pretreatment method for microchemical analysis of pectoral fin rays of Chinese sturgeon adopts the following process steps:

[0031] a. The Chinese sturgeon specimens were collected from the Changshu section of the Yangtze River in June 2015. After taking one of the specimens to measure the basic biological data, a section of the left pectoral fin ray was removed and washed with deionized water, and then the surface of the pectoral fin ray was further cleaned with pure alcohol To remove organic matter, put it into 18.2 MΩ.cm ultrapure water, and use an ultrasonic cleaning machine to ultrasonically clean it for 2~3 minutes. Finally, move the cleaned pectoral fin rays into a 24-well cell culture plate and dry it in an oven at 30~40°C. Dry for 8-10 hours, and finally move the pectoral fin rays into the cell culture plate and dry in the oven;

[0032] b. Prepare a small cuboid epoxy resin block, fix the base of the pectoral fin rays downward on the surface of the resin block

[0044] A pretreatment method for microchemical analysis of pectoral fin rays of Chinese sturgeon adopts the following process steps:

[0045] a. The Chinese sturgeon specimens were collected from the Changshu section of the Yangtze River in June 2015. After taking one of the specimens to measure the basic biological data, a section of the left pectoral fin ray was removed and washed with deionized water, and then the surface of the pectoral fin ray was further cleaned with pure alcohol To remove organic matter, put it into 18.2 MΩ.cm ultrapure water, and use an ultrasonic cleaning machine to ultrasonically clean it for 2~3 minutes. Finally, move the cleaned pectoral fin rays into a 24-well cell culture plate and dry it in an oven at 30~40°C. Dry for 8-10 hours, and finally move the pectoral fin rays into the cell culture plate and dry in the oven;

[0046] b. Prepare a small cuboid epoxy resin block, fix the base of the pectoral fin rays downward on the surface of the resin block

[0058] A pretreatment method for microchemical analysis of pectoral fin rays of Chinese sturgeon adopts the following process steps:

[0059] a. In June 2015, November 2015 and 2016, Chinese sturgeon specimens were collected from the Changshu section of the Yangtze River, the Yangtze River estuary, and the Jingzhou Chinese sturgeon artificial breeding base, and a section of the left pectoral fin was removed with deionized water. The surface of the pectoral fin rays was further cleaned with pure alcohol to remove organic matter, put it into ultrapure water of 18.2 MΩ.cm, and ultrasonically cleaned with an ultrasonic cleaner for 2-3 minutes. Finally, the cleaned pectoral fin rays were transferred to a 24-well cell culture plate. And dry it in a 30~40℃ oven for 8~10 hours, and finally move the pectoral fin rays into the cell culture plate and dry it in the oven;

[0060] b. Prepare a small cuboid epoxy resin block, fix the base of the pectoral fin rays downward on the surface of the