Culture medium for planting shiitake mushrooms, preparation method of culture medium and method for planting shiitake mushrooms

[0028] The preparation of the medium for planting shiitake mushrooms in embodiment A1~A3

[0029] Example A1

[0030] S11) Accurately weigh 70kg of water and 5kg of quicklime to prepare lime water;

[0031] S12) Accurately weigh 80kg of sawdust, 25kg of wheat bran, 20kg of rice bran and 4kg of corn flour and add them to the lime water obtained in S11, fully mix, let stand for 2 days, add HCl to adjust the Ph value to neutral;

[0032] S13) Sterilize the mixture obtained in S12 at 100° C. for 3 hours, and then cool to room temperature;

[0033] S14) Accurately weigh 0.5 kg of the strain of Bacillus megaterium and inoculate it into the mixture obtained in S13, and ferment in an aerobic environment at 38°C for 5 days;

[0034] S15) Accurately weigh 2kg of sucrose and 0.1kg of tannic acid and add to the mixture obtained in S14, and stir evenly;

[0035] S16) Accurately weigh 3kg of gypsum powder and add it into the mixture obtained in S15, and fully stir to obtain a culture medium

[0037] S11) Accurately weigh 85kg of water and 6kg of quicklime to prepare lime water;

[0038] S12) Accurately weigh 90kg of sawdust, 28kg of wheat bran, 23kg of rice bran and 5kg of corn flour and add them to the lime water obtained in S11, fully mix, let stand for 3 days, add HCl to adjust the Ph value to neutral;

[0039] S13) Sterilize the mixture obtained in S12 at 110° C. for 4 hours, and then cool to room temperature;

[0040] S14) Accurately weigh 0.8 kg of Bacillus colioides and inoculate into the mixture obtained in S13, and ferment in an aerobic environment at 40° C. for 8 days;

[0041] S15) Accurately weigh 3 kg of sucrose and 0.2 kg of adenine and add them to the mixture obtained in S14, and stir evenly; during specific implementation, adenine can also be replaced by pyrimidine;

[0042] S16) Accurately weigh 4kg of gypsum powder and add it into the mixture obtained in S15, and fully stir to obtain a culture medium for planting shiitake mushrooms.

[0044] S11) Accurately weigh 100kg of water and 8kg of quicklime to prepare lime water;

[0045] S12) Accurately weigh 100kg of sawdust, 30kg of wheat bran, 25kg of rice bran and 6kg of corn flour and add them to the lime water obtained in S11, fully mix them, let stand for 4 days, add HCl to adjust the Ph value to neutral;

[0046] S13) Sterilize the mixture obtained in S12 at 120° C. for 5 hours, and then cool to room temperature;

[0047] S14) Accurately weigh 1 kg of the strain of Streptomyces thermoamylase and inoculate it into the mixture obtained in S13, and ferment in an aerobic environment at 42° C. for 10 days;

[0048] S15) Accurately weigh 3 kg of sucrose and 0.2 kg of indole acetic acid and add them into the mixture obtained in S14, and stir evenly; during specific implementation, indole acetic acid can also be replaced by gibberellin;

[0049] S16) Accurately weigh 5kg of gypsum powder and add it into the mixture obtained in S15, and fully stir to obtain a culture