Specific primer and kit for rapid detection of candida albicans

[0024] Embodiment one:

[0025] 1. Primer design:

[0026] The nucleotide sequences of the specific primers used to detect Candida albicans are as follows:

[0027] Ft: 5'-TCTTTTTCGATGCGTACTGGACCAGCCGAGCCTTTCCTTCTGGGTAGC-3';

[0028] Bt: 5'-TGGTTTCTAGGACCATCGTAATGATTAATAGGGACGGTCG-3';

[0029] IF: 5'-AATTTGACTC-3';

[0030] IB: 5'-CCTTGGCCGAGAGGTCTG-3'.

[0031] 2. Kit design:

[0032] The kit includes reagent one, reagent two and blocking agent;

[0033] Wherein, reagent one comprises primer Ft, Bt, primer IF, IB, Bst DNA polymerase, dNTP and indicator;

[0034] Reagent two comprises Tris-HCl, ammonium sulfate, magnesium sulfate, potassium chloride, Tween-20 and betaine;

[0035] The sealing agent is paraffin.

[0036] Among them, the final concentration of each reagent in the kit is: Ft, Bt each 1.5μM, IF, IB each 0.9μM, dNTP each 1.5mM, Bst DNA polymerase 8U, Tris-HCl 20.0mM, ammonium sulfate 10mM, potassium chloride 50mM , Magnesium Sulfate 10mM, Tween-200.1%, Betain

[0037] Embodiment two:

[0038] 1. Primer design:

[0039] The nucleotide sequences of the specific primers used to detect Candida albicans are as follows:

[0040] Ft: 5'-TCTTTTTCGATGCGTACTGGACCAGCCGAGCCTTTCCTTCTGGGTAGC-3';

[0041] Bt: 5'-TGGTTTCTAGGACCATCGTAATGATTAATAGGGACGGTCG-3';

[0042] IF: 5'-AATTTGACTC-3';

[0043] IB: 5'-CCTTGGCCGAGAGGTCTG-3'.

[0044] 2. Kit design:

[0045] The kit includes reagent one, reagent two and blocking agent;

[0046] Wherein, reagent one comprises primer Ft, Bt, primer IF, IB, Bst DNA polymerase, dNTP and indicator;

[0047] Reagent two comprises Tris-HCl, ammonium sulfate, magnesium sulfate, potassium chloride, Tween-20 and betaine;

[0048] The sealing agent is paraffin.

[0049] Among them, the final concentration of each reagent in the kit is: Ft, Bt each 1.2μM, IF, IB each 0.7μM, dNTP each 1.2mM, Bst DNA polymerase 6U, Tris-HCl 20.0mM, ammonium sulfate 10mM, potassium chloride 50mM , Magnesium Sulfate 10mM, Tween-200.1%, Betain

[0050] Embodiment three:

[0051] 1. Primer design:

[0052] The nucleotide sequences of the specific primers used to detect Candida albicans are as follows:

[0053] Ft: 5'-TCTTTTTCGATGCGTACTGGACCAGCCGAGCCTTTCCTTCTGGGTAGC-3';

[0054] Bt: 5'-TGGTTTCTAGGACCATCGTAATGATTAATAGGGACGGTCG-3';

[0055] IF: 5'-AATTTGACTC-3';

[0056]IB: 5'-CCTTGGCCGAGAGGTCTG-3'.

[0057] 2. Kit design:

[0058] The kit includes reagent one, reagent two and blocking agent;

[0059] Wherein, reagent one comprises primer Ft, Bt, primer IF, IB, Bst DNA polymerase, dNTP and indicator;

[0060] Reagent two comprises Tris-HCl, ammonium sulfate, magnesium sulfate, potassium chloride, Tween-20 and betaine;

[0061] The sealing agent is paraffin.

[0062] Among them, the final concentration of each reagent in the kit is: Ft, Bt each 1μM, IF, IB each 0.5μM, dNTP each 1mM, BstDNA polymerase 6U, Tris-HCl 20.0mM, ammonium sulfate 10mM, potassium chloride 50mM, magnesium sulfate 10mM, Tween-200.1%, Betaine 0.6