Preparation method of hyaluronic acid cyclodextrin embedded collagen-anthocyanin pH indicating gel

[0045] Example 1

[0046] The preparation method of the hyaluronic acid cyclodextrin-embedded collagen-anthocyanin pH indicator gel of this embodiment is as follows:

[0047] 1. Preparation of hyaluronic acid cyclodextrin-embedded collagen-anthocyanin pH indicator gel:

[0048] a. Take 0.09g of food-grade hyaluronic acid and dissolve it in physiological saline at 4°C with a mass fraction of 0.9% (w / v). Stir it with a magnetic stirrer at 100rpm at 4°C until it is completely dissolved. And adjust the pH of the gel to 5.4~6.2 with 0.1mol / L hydrochloric acid solution as needed to form a transparent and non-flowing gel;

[0049] b. Extract anthocyanins from perilla leaves, peony flowers and snake fruit peels with cellulase-pectinase combined assisted ultrasonic method. Take appropriate amount of perilla leaves, peony flowers and snake fruit peels and dry them at 50°C for 2 hours, then mill , Take 2g of the mixed powder to a 100mL Erlenmeyer flask with stopper, add 0.05g pectinase, 0.05g

[0052] Example 2

[0053] Apply the gels ①, ②, ③, and ④ prepared in Example 1 to the preservation and instructions of crucian carp:

[0054] Preservation of crucian carp and instructions

[0055] Each purchased fresh crucian carp weighing about 1000g is transported to the laboratory in a foam box filled with ice cubes within 1 hour, and quickly divided on crushed ice, heading, gutting, tailing, and skinning are removed. The trunk is evenly divided into upper and lower parts from the ventral fin to the dorsal fin, about 15cm long and 8cm wide. Rinse it quickly with running water and absorb it with filter paper. Apply the prepared gel to the surface of the divided fish completely and evenly, and dry the surface. A layer of milky white, opaque, uniform protective film is formed. Store at 4°C to monitor the discoloration and state changes of the gel. The result is figure 1 Shown by figure 1 It can be concluded that with the extension of storage time, the pH of crucian carp meat gradually

[0057] Example 3

[0058] The preparation method of the hyaluronic acid cyclodextrin-embedded collagen-anthocyanin pH indicator gel of this example is different from Example 1 in that the following collagens are respectively embedded: ① catfish skin collagen; ② Catfish skin collagen; ③ horse noodle fish skin collagen; ④ tilapia skin collagen; ⑤ shark skin collagen, the others are the same as in Example 1.