Chloroplast SSR (simple sequence repeat) labeled primers for molecular identification of compositae plants and acquisition method of chloroplast SSR labeled primers

[0036] Example 1

[0037] Chloroplast SSR marker primers identified by a group of Askichi plant molecules, the chloroplast SSR labeled primer is one or more of SSR1, SSR2 or SSR3;

[0038] The primer sequence of SSR1 tags is:

[0039] Upstream primer sequence ssr1_f: 5'-ccaacgagtcacactaagc-3 ';

[0040] Downstream primer sequence ssr1_r: 5'-cttTgcattctatcccAgcga-3 ';

[0041] The primer sequence of SSR2 tags is:

[0042] Upstream primer sequence SSR2_F: 5'-gttttctcctcgtacggctC-3 ';

[0043] Downstream primer sequence SSR2_R: 5'-ccacttcaattgtctCacgg-3 ';

[0044] The primer sequence of SSR3 tags is:

[0045] Upstream primer sequence ssr3_f: 5'-cgaaccacgctttttcta-3 ';

[0046] The downstream primer sequence SSR3_R: 5'-atgctgcagttgtgattgat-3 '.

[0047] Example 2

[0048] A method of acquiring a chloroplast SSR marker primer identified as described in Example 1, such as figure 1 As shown, including the following steps.

[0049] (1) Retrieve and obtain the chloroplast genome.

[0050] Specifically, asteraceae is keywords to retrieve the chloroplast genome public database CPBase database (address: https: / / rocaplab.ocean.washington.edu / tools / cpbase / ) and NCBI Caineer Genomic Database (Address: https: / / www.ncbi.nlm.nih.gov / genome / organelle / , a total of 136 astrikhoid genome, then download and organize the genome sequence to a FASTA format file, used for downstream SSR sites Identification.

[0051] (2) Predict and obtain the identified Askry Chloroplast genome SSR site to develop SSR tags.

[0052]Specifically, 136 astriper genome sequences obtained by step (1), using the MISA software (download address: http: / / pgrc.ipk-gatersleben.de / misa / misa.html) to perform SSR bit prediction And identification,

[0077] Example 3

[0078] The acquisition method of the chloroplast SSR molecular marker primer identified by the chrykem species molecule as provided in Example 2 is of great significance in the identification of the species molecular identification.

[0079] First, the acquisition method retrieves 136 astringent leaf genome sequences, including 40 genus, which is the most comprehensive synacoochemical genome sequence information, which has strong representative.

[0080] Second, the acquisition method is based on the biological information analysis method, using the SSR site in the obtained Juku leaf genome, and is designed to develop SSR primers by using the MISA software and Primer 3.0 tools and PRIMER3.0 tools. Further, a method of electron PCR is further utilized, based on the BLAST sequence alignment, the development of the SSR primer is compared and screened in this 136 astriper leaf genome, and the SSR mark of high polymorphism is obtained. Thus, using the technical system,