Application of providencia bacterial strain in preparation of divalent manganese oxidant

A technology of divalent manganese and oxidant, which is applied in the fields of microorganism application and environmental restoration, which can solve the problems of insufficient adsorption capacity of heavy metals and degradation of organic matter, and achieve the effect of overcoming harsh reaction conditions

Pending Publication Date: 2022-01-21
HUNAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical effect of this patented technology involves making use of specialized microorganisms called pLoberius bacillis that can break down certain metal ions like iron or copper from polluted waters such as lakes and rivers. This process makes them easier than current methods but also has lower costs compared with traditional chemicals due to its ability to be easily prepared at home without requiring complicated operations.

Problems solved by technology

The technical problem addressed in this patented text relates to finding ways to reduce or remove contamination caused by certain substances that may affect humans and animals due to its negative impact on both animal reproduction and growth performance. This includes chemical agents like chromate compounds used during fertilization processes, phosphorus containing materials released into our atmosphere after combating crops, nitrogenous wastes produced when manufacturing plastic containers made from these polymers, and even small amounts of dyes added onto rubber tires. These methods aim at reducing or removing unwanted elements while maintaining beneficial properties found naturally within an indoor community.

Method used

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  • Application of providencia bacterial strain in preparation of divalent manganese oxidant
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  • Application of providencia bacterial strain in preparation of divalent manganese oxidant

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Embodiment Construction

[0033] The bacterial strain of the genus Providence provided by the present invention is preserved in China Center for Type Culture Collection, Wuhan, China, and the preservation number is CCTCC NO: M 2018876.

[0034] 1. Identification of Providencia sp.LLDRA6 Mn(II) oxidation ability and purification of manganese trioxide.

[0035] 1) Main reagents:

[0036] ① LBB indicator; ② Reagent 1: bacteria powder LLDRA6; ③ Reagent 2: phenol; ④ Reagent 3: chloroform; ⑤ Reagent 4: methanol; ⑥ Reagent 5: sodium hypochlorite.

[0037] 2) Solution configuration

[0038] ① LB medium: 5g yeast extract powder, 10g peptone, 5g sodium chloride, 1L deionized water, pH to 7.2-7.4.

[0039]② LBB preparation: first weigh 40mg of LBB drug powder, and then dissolve it in the acetic acid solution with a concentration of 45mM prepared in advance. After the dissolution is complete, transfer it to a 100mL volumetric flask for constant volume. The mass fraction of the prepared LBB solution is 0.04%. Fina

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Abstract

The invention discloses an application of a providencia bacterial strain in preparation of a divalent manganese oxidant. The providencia bacterial strain can also be applied to degradation of atrazine in polluted water body. The invention further provides a kit and a method for economically and simply preparing manganese oxide, thereby overcoming defects of hostile reaction conditions, complicated operations, high cost and low efficiency in existing chemical synthesis technologies of manganese oxide.

Description

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Claims

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Application Information

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Owner HUNAN UNIV OF TECH
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