Preparation method of antiviral specific T cell

[0044] Example 1 Effect of high concentration of potassium chloride against a proportion of taletic virus specific T cells

[0045] (1) Take the HLA-A * 02: 01 gene screening results as positive and peripheral blood in patients with positive patients, this example involves 1 patient, patients with hematopoietic stem cell transplantation giant cell virus positive patients, selected from Henan Provincial Cancer Hospital, this experiment has been licensed.

[0046] (2) The peripheral blood of step (1) is obtained by FICOLL density gradient method to obtain a single nuclear cell.

[0047] (3) The single nuclear cells separated by step (2) are divided into five groups, and the five groups of cells are resuspended by the following culture fluids:

[0048] 1 Do not add potassium chlorinated serum culture solution (0g / L), add human IL-2 (final concentration is 10 6 U / L);

[0049] 2 Add 1 g / L of potassium chlorinated liquor medium, add human IL-2 (final concentration is 10 6 U /

[0057] Example 2 Effect of high concentration of potassium chloride on the proportion of antiviral specific T cells in PD-1 positive T cell subsets

[0058] (1) Take HLA-A * 02: 01 gene screening results positive and peripheral blood in patients with giant cytological virus positive patients, in this example, in this example, the patient is a patient with hematopoietic stem cell transplantation giant cell virus positive patients, selected from Henan Province Tumor hospitals, this experiment has been licensed.

[0059] (2) The peripheral blood of step (1) is obtained by FICOLL density gradient method to obtain a single nuclear cell.

[0060] (3) A single nuclear cell obtained by step (2) is first incubated with anti-human PD-1 antibody (IgG4 subtype) at 4 ° C for 10 min, and then incubated with biotinylated anti-human IgG4 antibody at 25 ° C. After 15 min, the antibiotic antibody coated magnetic bead was incubated at 4 ° C for 10 min, and the magnetic force was separated to obtain a s

[0067] Example 3 Effect of high concentration of potassium chloride against EB virus specific T cell ratio

[0068] (1) Take the HLA-A * 02: 01 gene screening results, the peripheral blood of the EB virus positive patients, the present embodiment involves 5 patients, selected from the Henan Tumor Hospital, this experiment has been licensed.

[0069] (2) The peripheral blood of step (1) is obtained by FICOLL density gradient method to obtain a single nuclear cell.

[0070] (3) The single nuclear cells separated by step (2) are divided into five groups, and the five groups of cells are resuspended by the following culture fluids:

[0071] 1 Do not add potassium chlorinated serum culture solution (0g / L), add human IL-2 (final concentration is 10 6 U / L);

[0072] 2 Add 1 g / L of potassium chlorinated liquor medium, add human IL-2 (final concentration is 10 6 U / L);

[0073] 3 Add 2 g / L of potassium chloride has no serum culture solution, add human IL-2 (final concentrat