Method for manufacturing calibration color card of newborn percutaneous jaundice instrument

[0031] A kind of neonatal percutaneous jaundice meter calibration color card manufacturing method, comprising the following steps:

[0032] A. Solution preparation

[0033] 1) Prepare bilirubin solutions with concentrations of 1, 5, 11, and 17 mg / dl with chloroform and bilirubin dry powder;

[0034] 3) measure the spectral characteristics of the bilirubin solution with a spectrophotometer; according to Lambert Beer's law A=lg(1 / T)=Kbc, find the actual molar absorption coefficient of the bilirubin solution, and fit the bilirubin solution The relationship between the concentration and the measured value, and draw the curve.

[0035] B. Mold making

[0036] 1) Make a set of disc-shaped injection molds with a diameter of 50mm and a thickness of 2mm;

[0037] C. Production of color cards

[0038] 1) Using toner according to a certain ratio, mixed with plastic pellets for injection molding processing, to obtain a color card for injection molding;

[0039] 2) Analyze the wafer

[0044] The calculation method of the concentration of the bilirubin solution corresponding to the color card is:

[0045] 1) Put the color card into the spectrophotometer, and the spectrophotometer measures the transmittance of the color card at wavelengths of 453nm and 560nm, denoted as A 453 and A 560 ;

[0046] 2) Use a micrometer to measure the actual thickness of the color card, and record it as L;

[0047] 3) Calculate the bilirubin concentration value corresponding to the color card according to Lambert-Beer's law:

[0048] ln(A 453 / A 560 )=-K*C*L

[0049] C=ln(A 453 / A 560 ) / K*L

[0050] Wherein, K is the blue-green light absorbance measured by the spectrophotometer of the bilirubin solution, obtained by Lambert-Beer's law A=lg(1 / T)=Kbc.

[0051] The calibration value of the jaundice meter is obtained by converting the bilirubin concentration value: 1mg / dl=17.1umol / L.

[0052] Through the injection molding process, the present invention ensures that