Kit and device for ATRX and KDM5A mutation detection based on digital PCR technology and application

[0023] The preparation method of described sizing agent is:

[0024] Mix 5-10 parts of sodium lauryl sulfate, 1-3 parts of hydrochloric acid and 1-5 parts of chitosan solution, the mixing speed is 100-300r / min, the mixing time is 10-20min, and the mixing is completed;

[0025] Then add 1-5 parts of modified silica, continue to stir at a speed of 100-200rmin for 20-30min, the stirring is completed, and then add 1-3 parts of modified wollastonite to obtain a sizing agent.

[0026] The modification method of the modified silica in this embodiment is as follows: the silica is sent to 100-300°C for calcination for 10-20min, the calcination is finished, then the temperature is changed to 50-60°C, and then sent to water for static Set aside and cool to room temperature.

[0027] The specific operation steps of the temperature-change-down treatment in this embodiment are: dropping to 50-60°C at a rate of 1-5°C / min.

[0028] The mass fraction of the chitosan solution in this embodim

[0035] The kit for detecting ATRX and KDM5A mutations based on digital PCR technology in this embodiment includes mutant probes, infiltrating agents and additives in a weight ratio of 4:2:1;

[0036] The preparation method of described sizing agent is:

[0037] Mix 5 parts of sodium lauryl sulfate, 1 part of hydrochloric acid and 1 part of chitosan solution, the mixing speed is 100r / min, the mixing time is 10min, and the mixing is completed;

[0038] Then, 1 part of modified silica was added, and the stirring was continued for 20 min at a rotating speed of 100 rmin, and the stirring was completed, and then 1 part of modified wollastonite was added to obtain a sizing agent.

[0039] The modification method of the modified silica in this embodiment is as follows: the silica is sent to 100°C for calcination for 10min, the calcination is completed, then the temperature is changed to 50°C, then put into water and left to stand, and cooled to room temperature .

[0040] The specifi

[0047] The kit for detecting ATRX and KDM5A mutation based on digital PCR technology in this embodiment includes mutant probe, infiltrating agent and additives in a weight ratio of 4:2:1;

[0048] The preparation method of described sizing agent is:

[0049] Mix 10 parts of sodium lauryl sulfate, 3 parts of hydrochloric acid and 5 parts of chitosan solution, the mixing speed is 300r / min, the mixing time is 20min, and the mixing ends;

[0050] Then, 5 parts of modified silica were added, and the stirring was continued for 30 minutes at a rotating speed of 200 rmin, and the stirring was completed, and then 3 parts of modified wollastonite were added to obtain a sizing agent.

[0051] The modification method of the modified silica in this example is as follows: the silica is sent to 300°C for calcination for 20min, the calcination is completed, then the temperature is changed to 60°C, then put into water and left to stand, and cooled to room temperature.

[0052] The specific ope