Glutathione reductase determination kit

A technology of glutathione and oxidized glutathione, applied in the field of biochemical detection, can solve the problems of complex dispensing, quality control and high cost of glutathione reductase assay kits, and achieve the goal of being suitable for clinical application and The effect of generalization, good stability and wide linear range

Pending Publication Date: 2022-07-22
江西英大生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Judging from the above-mentioned prior art reports, the glutathione reductase as

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1:

[0026] A glutathione reductase assay kit, comprising a reagent R1 and a reagent R2, wherein the reagent R1 comprises: Tris buffer 50mmol / L, ascorbate oxidase 1KU / L, pyruvate oxidase 1KU / L, ferrocyanide Potassium chloride 5mg / L, oxidized glutathione GSSG 2mmol / L, MgCl 2 1mmol / L and alkyl glycoside APG0810 1g / L. Reagent R2 includes: Tris buffer 50 mmol / L, NADPH 0.1 mmol / L and alkyl glycoside APG0810 1 g / L.

Example Embodiment

[0027] Example 2:

[0028] A glutathione reductase assay kit, comprising a reagent R1 and a reagent R2, wherein the reagent R1 comprises: Tris buffer 50mmol / L, ascorbate oxidase 1KU / L, pyruvate oxidase 1KU / L, ferrocyanide Potassium chloride 5mg / L, oxidized glutathione GSSG 2mmol / L, MgCl 21mmol / L and alkyl glycoside APG1214 1g / L. Reagent R2 includes: Tris buffer 50mmol / L, NADPH 0.1mmol / L and alkyl glycoside APG1214 1g / L.

Example Embodiment

[0029] Example 3:

[0030] A glutathione reductase assay kit, comprising a reagent R1 and a reagent R2, wherein the reagent R1 comprises: Tris buffer 50mmol / L, ascorbate oxidase 1KU / L, pyruvate oxidase 1KU / L, ferrocyanide Potassium chloride 5mg / L, oxidized glutathione GSSG 2mmol / L, MgCl 2 1mmol / L and alkyl glycoside APG0814 1g / L. Reagent R2 includes: Tris buffer 50mmol / L, NADPH 0.1mmol / L and alkyl glycoside APG0814 1g / L.

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Abstract

The invention discloses a glutathione reductase determination kit, which comprises a reagent R1 and a reagent R2, and is characterized in that the reagent R1 comprises a Tris buffer solution, ascorbic acid oxidase, pyruvate oxidase, potassium ferrocyanide, oxidized glutathione GSSG, MgCl2 and alkyl glycoside; the reagent R2 is prepared from a Tris buffer solution, NADPH (Nicotinamide Adenine Dinucleotide Phosphate) and alkyl glycoside. According to the present invention, the alkyl glycoside is added to the reagent R1 and the reagent R2 of the glutathione reductase determination kit, such that the kit has characteristics of good stability and wide linear range, can achieve the accurate determination of glutathione reductase, and is suitable for clinical application and promotion.

Description

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Claims

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Application Information

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Owner 江西英大生物技术有限公司
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