The invention discloses an olive leaf and grape botanical pesticide. The pesticide is prepared from olive leaves, momordica cochinchinensis, melia azedarach leaves, sweet-clover astragalus, agrimonia pilosa ledeb, sanguisorba officinalis, Chinese prickly ash, cinnamon, cortex phellodendri, atractylodes macrocephala koidz and a modified starch solution. Main components of the pesticide are extracted from Chinese herbal medicine. Compared with a traditional organic pesticide, the pesticide has the advantages of being safe, low in toxin and free of pungent smell and pesticide resistance, and after reasonable complexing, the antibacterial and insect-killing effects are remarkable; by adding modified starch, the pesticide is better in stability and difficult to decompose; meanwhile, the modified starch can adsorb excessive components in the pesticide, and pesticide residues of fruits are further reduced; besides, the extraction components of Chinese herbal medicine contain various nutrients and also contain active enzyme, antioxidant, growth promoting factors and the like, quality of grapes is improved, the seedless rate of grapes is increased, and the effect of reducing the cholesterol level of a human body is enhanced.