The invention provides an organic semiconductor material, the chemical formula of which is shown as the following. The organic semiconductor material contains triphenylamine, carboline and pyrene. Specifically, the triphenylamine has hole injection ability and transport property, the carboline has good electronic transport performance, and the pyrene has very high fluorescence quantum yield. Thus, the organic semiconductor material provided by the invention is a bipolar blue luminescent material with good hole transport and electronic transport abilities. The invention also provides a preparation method of the organic semiconductor material, and an electroluminescent device including the organic semiconductor material.