Polar dyes

Inactive Publication Date: 2006-10-12
CEPHEID INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0146] In another aspect, the present invention provides method of directly detecting a target nucleic acid, comprising: providing a dye-labeled oligonucleotide probe comprising an oligonucleotide conjugated to one or more dye compounds of Formulae (I-III) and (V-VIII), the oligonucleotide further optionally comprising an MGB optionally substituted with one or m

Problems solved by technology

The excited-state energy of the donor fluorophore is transferred to the neighboring acceptor by a resonance dipole-induced dipole interaction, which results in quenching of the donor fluorescence.
Fluorescent dyes and quenchers, however, may substantially change the physical properties

Method used

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Examples

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example 1

[0535] Example 1 is a phosphoramidite dye reagent of Formula (II) having an arsonate ester. The dye reagent is suitable for automated oligonucleotide synthesis using the phosphoramidite method.

The dye reagent was prepared according to Methods C and D.

example 2

[0536] Example 2 shows a carboxylic acid dye reagent of Formula (III) having two arsonate ortho esters. The dye reagent is suitable for attaching to a solid support such as CPG to initiate an oligonucleotide synthesis.

[0537] The dye reagent was prepared according to Methods B and D.

example 3

[0538] Example 3 shows a phosphoramidite dye reagent of Formula (III) having two arsonate diesters employing pinacol as a protective chelating agent. The dye reagent is suitable for automated oligonucleotide synthesis using the phosphoramidite method.

[0539] The dye reagent was prepared according to Methods A, C, D and E. More detailed synthetic pathway is further illustrated in FIG. 4.

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Abstract

The present invention relates to novel polar fluorescent and quenchers dyes, and minor groove binder with enhanced polarity. The present invention further relates to methods of preparing oligonucleotide probes labeled with polar arsonate dyes under the condition of automated synthesis and method of using such probes.

Description

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Claims

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Application Information

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Owner CEPHEID INC
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