Measurement kit and an immunochromatography method

(1) Preparation of Immunochromatography Kit for Detection of Influenza Types A and B

(1-1) Preparation of Anti-Influenza Types A and B Antibodies-Modified (Immobilized) Gold Colloids

(1-1-1) Preparation of Anti-Influenza Type A Antibody-Modified (Immobilized) Gold Colloid

[0225]1 mL of a 90 μg / mL anti-influenza type A monoclonal antibody (Anti-Influenza A SPTN-5 7307, Medix Biochemica) solution was added to a gold colloidal solution having pH adjusted by addition of 1 mL of a 50 mM KH2PO4 buffer (pH 7.5) to 9 mL of a 50-nm diameter gold colloidal solution (EM.GC50, BBI), followed by agitation. The mixture was allowed to stand for 10 minutes and then 550 μL of 1% polyethylene glycol (PEG Mw. 20000, Product No. 168-11285, Wako Pure Chemical Industries, Ltd.) aqueous solution was added to the mixture, followed by agitation. A 10% bovine serum albumin (BSA Fraction V, Product No. A-7906, SIGMA) aqueous solution (1.1 mL) was added to the resultant, followed by agitation. The solution was cen

Amplification from 30-Degree Direction

[0242]The kit prepared in (1-5) above was used such that the angle between the developing direction of the amplification solution and the developing direction of the sample solution became 30 degrees. Addition of an antigen solution was carried out in the same manner as that described in (2-1) above. Ten minutes after the addition of the antigen solution, the amplification solution addition pad was immersed in a plastic tray containing 40 mL of the amplification solution A prepared in (1-6) above, and silver amplification was then carried out for 2 minutes. Thereafter, this membrane was removed from the amplification solution, and it was then well washed with water for 3 minutes. Thereafter, the membrane was subjected to measurement using a concentration measurement apparatus for immunochromatography (ICA-1000; Hamamatsu Photonics K.K.) so as to measure an optical concentration reflecting to a reference white board. This measurement was carried out

Amplification from 45-Degree Direction

[0244]The kit prepared in (1-5) above was used such that the angle between the developing direction of the amplification solution and the developing direction of the sample solution became 45 degrees. Except for this, the experiment was carried out in the same manner as that described in .