Safe delivery of crispr and other gene therapies to large fractions of somatic cells in humans and animals

DESCRIPTION OF EMBODIMENT

The Preferred Embodiment of Part 1

[0070]The preferred embodiment of Part 1 of the invention is a method comprising delivery of drugs to inhibit the immune system, the method comprising the steps.[0071]a. An inhibitor of NFκB.[0072]b. An inhibitor or IRF3, TLR3, TLR4, and TLR7 / 8. The SSRI, sertraline, is preferred due to lower toxicity. Other SSRI or SNRI drugs may also prove to work for this purpose.[0073]c. A TLR9 inhibitor if it is required.[0074]d. A TNFα inhibitor.[0075]e. An mTOR inhibitor.[0076]f. An NADV for delivery of nucleic acid into cells of a living animal.

The Preferred Embodiment of Part 2

[0077]The preferred embodiment of Part 2 of the invention is a structure comprising:[0078]a. An expression vector plasmid containing CpG-ODNs.[0079]b. An expression vector plasmid with a promoter dependent on a TetR system.[0080]c. An expression vector plasmid with a promoter, and multiple protein coding sequences with CHYSEL linkers between them.