The invention discloses a precious metal anti-cancer nano material and a preparation method and application thereof.The preparation method comprises the steps that a chloroauric acid aqueous solution, a chloroplatinic acid aqueous solution and a copper chloride aqueous solution are evenly mixed, an ascorbic acid aqueous solution is added, heating is conducted for 1-4 h at the temperature of 30-60 DEG C, a product is centrifugally washed, and the precious metal anti-cancer nano material is obtained. The precious metal anti-cancer nano material with the face-centered cubic structure can be obtained. According to the preparation method of the precious metal anti-cancer nano material, the problem that in the prior art, the preparation conditions for preparing the precious metal-transition metal alloy nano material are harsh and tedious is solved. And the reaction is carried out in a water phase without adding an organic solvent or a surfactant, so that the prepared precious metal anticancer nano material can be prevented from being polluted by reagents.