The invention discloses a method for determining
methamidophos in rice
wine and particularly discloses a method for detecting the content of
methamidophos in rice
wine by utilizing
gas chromatography and liquid
chromatography-
tandem mass spectrometry. The method comprises the following steps: (1) extraction and purification: utilizing a
solid phase extraction column to extract the
methamidophos in the rice
wine and using
ethyl acetate to elute the methamidophos adsorbed on the
solid phase extraction column; (2) preparation of matrix matched standard working solution; (3) qualitative screening and quantification by utilizing
gas chromatography; (4) qualitative confirmation of positive samples by using liquid
chromatography-
tandem mass spectrometry; (5) blank test; and (6) result calculation and representation. The method has the following beneficial effects: the pretreatment operation is simple and practical, and the
recovery rate of the methamidophos is high; and by adopting the matrix matched standard working solution, the problem of matrix effect of the methamidophos on the gas chromatograph is solved, and accurate quantification is implemented.