The invention discloses
hyaluronic acid-based modified
gliadin nanoparticles as well as a preparation method and application thereof. The method for preparing the W2 / O2 / (O1 / W1) type multiple emulsionfrom the nanoparticles comprises the following steps: mixing polyglycerol ricinoleate with
corn oil at 65 DEG C to form a first
oil phase, mixing a
mixed solution of
sodium chloride and
gelatin with the obtained first
oil phase, and performing homogenizing by adopting high-pressure microjet to obtain a W2 / O2 type primary
emulsion; mixing a second
oil phase with the
nanoparticle suspension, and performing high-shear treatment to obtain an O1 / W1 type
Pickering emulsion; and mixing the W2 / O2 type primary
emulsion with the O1 / W1 type
Pickering emulsion by adopting a high-pressure homogenization technology, so as to prepare the W2 / O2 / (O1 / W1) type multiple
emulsion. The preparation method of the multiple emulsion is simple, mild in reaction condition and suitable for industrial production, and can be applied to the fields of foods, medicines,
cosmetics and the like.