The invention discloses a novel charged nanofiltration membrane for removing organic matter and a preparation method of the novel charged nanofiltration membrane. The preparation method comprises the following steps: (1) dissolving polysulfone resin into a mixture of DMF and ethylene glycol, and conducting stirring and degassing to obtain a resin membrane casting solution; (2) coating a non-woven fabric with the resin membrane casting solution, and performing phase inversion in deionized water to form pores, so as to obtain a porous polysulfone-based membrane; (3) preparing any one of a disodium hydrogen phosphate-citric acid buffer solution, a citric acid-sodium citrate buffer solution or an acetic acid-sodium acetate buffer solution; (4) adding chitosan, a piperazine monomer and an anti-pollution additive into the buffer solution, and uniformly conducting stirring to obtain a water-phase solution; (5) mixing trimesoyl chloride and isophthaloyl chloride, adding the mixture into an organic solvent, and uniformly conducting stirring to obtain an oil phase liquid; (6) cutting the porous polysulfone-based membrane, soaking the porous polysulfone-based membrane in the water-phase liquid, and removing the water-phase liquid on the surface of the porous polysulfone-based membrane after soaking; (7) conducting polymerizing in the oil phase liquid after soaking; and (8) after polymerization, conducting drying to obtain the novel charged nanofiltration membrane for organic matter removal.