The invention relates to a technology for detecting the content of active ingredients of a disinfectant, in particular to test paper for determining the content of chlorine dioxide and a preparation method thereof. The test paper comprises a test paper substrate and a test paper detection base layer adhered to one end of the test paper substrate, and the test paper detection base layer is coated with a reaction substrate which is colorless and develops color after being in contact with chlorine dioxide. Compared with the prior art, a solution method is converted into a test paper method, a specific environment and professional operation skills are not needed, and when detection is needed, rapid detection can be achieved, the detection speed is high, the color gradation of a detection result is clear, indication is accurate, and use is convenient; the test paper for rapidly detecting the concentration of the chlorine dioxide disinfectant can be used in the industries of medical treatment and public health, food processing, catering and the like, and has the characteristics of accurate concentration indication, clear color gradation of different concentrations, simplicity and convenience in operation, high test speed, no toxicity to a human body, high safety and the like.