The invention discloses a test strip for detecting an organophosphorus pesticide and application thereof. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reactionfilm (3), a water absorbent pad (4) and a bottom plate (7), and the reaction film comprises a detection line (5) coated with an organophosphorus pesticide hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse antibody, the conjugate release pad (2) is sprayed with an organophosphorus pesticide monoclonal antibody-colloidal gold label. The invention also provides a method for detecting organophosphorus pesticide residues in crops such as vegetables and fruits by using the test strip for detecting the organophosphorus pesticide. The test stripn has the characteristics of simple operation, high sensitivity, fast detection speed and low cost, and is suitable for screening and on-site monitoring of a large number of samples.