1 results about "Spermatogonial stem cells" patented technology

The invention discloses a method for obtaining transgenic chickens through spermatogonial-stem-cell in vivo transfection mediated by lentivirus vectors. The method comprises the steps of directly injecting a lentivirus vector into a testis of a cock, transferring genes of endogenous spermatogonial stem cells to generate transgenic sperms, mating the cock with a normal hen and then obtaining the transgenic chickens. Through the way with the endogenous spermatogonial stem cells, the method for obtaining transgenic chickens through spermatogonial-stem-cell in vivo transfection mediated by lentivirus vectors can avoid opening poultry eggs, and has the advantages of low virus consumption, fast group extension and capability of stable passage.