The invention aims to provide a method for improving euryops pectinatus oil aroma quality and application thereof. The method includes: subjecting euryops pectinatus to low temperature superfine comminution, then adding a certain amount of acid, an oxidant and enzyme and mixing the substances evenly in an aqueous solution, conducting incubation fermentation for a certain period of time under a synergistic effect, and carrying out steam distillation to extract volatile oil. The method improves the extraction rate and aroma quality of euryops pectinatus oil, the euryops pectinatus oil has composite, natural and rich aroma, besides chrysanthemum characteristic aroma, also has flower fragrance, fruit sweet aroma, slightly cool sense feel and other natural smell, the irritation is reduced, andthe aroma quality is improved. The prepared euryops pectinatus oil can be applied in cigarette formula cut tobacco, reconstituted tobacco, and cut stems, the prepared cigarette has clear aroma, chrysanthemum characteristic note, rich and pure aroma, and clean aftertaste, and overcomes the defects of too strong chrysanthemum characteristic aroma, increased irritation, little miscellaneous qi, and slightly bitter aftertaste.