4 results about "Transcriptome" patented technology

The invention relates to the technical field of biological information, in particular to a method for analyzing and mining functional genes by combining genome three-dimensional structure difference identification and transcriptome gene expression level difference. The invention provides a genome three-dimensional structure difference identification method, which comprises identification of difference AB Compartment, identification of difference TAD and identification of difference Loop on the basis of HiC data, and can realize efficient identification of different levels of genome three-dimensional structure differences of animals and plants by using in-situ HiC data. The invention provides a method for analyzing and excavating functional genes by utilizing the genome three-dimensional structure difference identification method in combination with transcriptome gene expression level difference, and by utilizing the combined analysis method, difference analysis can be performed from two aspects of a genome three-dimensional structure and a transcriptome, so that efficient excavation of animal and plant functional genes is realized.

The invention relates to a screening method of silkworm hemocyte specific expressed genes and a method for analyzing and finding a hemocyte specific expression regulation element through cloning of a protease O gene promoter. The hemocyte specific expression regulation element is manufactured through the following steps that 1, a hemocyte specific expression candidate gene protease O gene is screened out through silkworm tissue chip expression data, transcriptome data, a quantitive RT-PCR and other methods, and the silkworm hemocyte specific expression of the gene is verified successfully; 2, a related gene promoter is successfully cloned, and functional analysis is carried out; 3, a Bac-to-Bac rhabdovirus expression system is successfully improved, an effective hemocyte promoter detection system is built, the activity of the candidate gene promoter is detected at the cell and the individual level, and tissue specificity of the promoter is verified; 4, the silkworm hemocyte specific gene protease O gene promoter is obtained through the study, and it is analyzed and found that the promoter is provided with the tissue specificity regulation element in the section between the upstream -333 and -80 of a transcriptional start site. The specificity expression of the gene on hemocyte is controlled.

The invention falls within the fields of biochemistry, molecular biology, genetics, pharmacy and medicinal chemistry, being related to biochemical and metabolic processes. More specifically, the present invention provides a novel class of proteins that inhibit thrombin, particularly direct inhibitors of thrombin modified from sculptin, identified in the transcriptomics analysis of the salivary glands of ticks, as well as fragments and recombinant protein thereof, which can be used as anticoagulant agents and for the prophylaxis and/or treatment of thromboembolic diseases.

The invention discloses a reference gene for stable expression of red wheat blossom midges in different growth periods and application of the reference gene. The nucleotide sequence of the reference gene RPS18 is as shown in SEQ ID NO: 1. The related gene sequence of the red wheat blossom midges is obtained according to measured red wheat blossom midge transcriptome data, a primer is designed, and six gene nucleotide sequence of the red wheat blossom midges is obtained by PCR reaction. The red wheat blossom midges under different development conditions are used as experimental materials to carry out Real time PCR detection, Ct data are analyzed by geNorm reference gene stability analyzing software, the reference gene RPS18 with the most stable expression of the red wheat blossom midges under the different development conditions is screened out, therefore, reference is provided for quantification of the gene of the red wheat blossom midges under the condition of the follow-up different development periods, and therefore, accuracy of the result of quantitative experiments is guaranteed.