Method for degrading sterol compounds in tobacco extract with biological method
A technology of leachate and sterols, applied in the field of sterols harmful compounds, can solve the problems of loss of fragrance substances and latent substances, few sterols, and unsatisfactory effects, etc., and achieves high originality, simple and fast process. Effect
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[0023] A biological method for degrading sterols in tobacco leachate, and its steps are as follows:
[0024] (1) Isolation of sterol-lowering microorganisms: Tobacco leaves are from Xiang County, Henan Province. After the tobacco leaves are destemmed, the leaves are cut into 2cm*2cm small leaves, washed twice with sterile deionized water, and the precipitate is collected and used 15ml pH7.0 Dilute the precipitate with phosphate buffer solution, inoculate it into the separation medium according to the 10% inoculum amount, incubate in a shaker at 37°C at 150r / min, and after enrichment for 2 times, take 200μl of the culture solution and apply it on the solid plate. A single colony of microorganisms that can degrade sterols. The raw materials of the separation medium are: stigmasterol 7412.1g; sorbitan monooleate polyoxyethylene ether (tween80) 0.9ml; citric acid 2.1g; K 2 HPO 4 0.4g; NH 4 Cl 1.1g; add water to 0.9L.
[0025] (2) Microbial expansion culture: pick the single colony in s
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