Method for determining content of caulis spatholobi in Kangfuling capsule

[0061] Example 1 Determination of the content of Caulis spatholobi in Kangfuling Capsules: According to Appendix VID of the 2010 edition of the Chinese Pharmacopoeia, determination:

[0062] Chromatographic conditions and system adaptability test: octadecylsilane bonded silica gel is used as a filler, acetonitrile-water=35:65 is used as the mobile phase, and the detection wavelength is 254nm;

[0063] Preparation of reference substance solution: take formononetin reference substance, dissolve it in 80% methanol, and make a solution containing 0.03mg formononetin reference substance per 1 mL as a reference substance solution;

[0064] Preparation of the test solution: Take 5g of the contents under the difference in the amount of this preparation, add 50mL of 80% methanol, and extract with reflux in a water bath for 30 minutes, filter, evaporate the filtrate to dryness, add 10mL of 80% methanol to the residue to dissolve, filter Once, take the continued filtrate as the test solution;

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[0066] Example 2 Determination of the content of Caulis spatholobi in Kangfuling Capsules: According to Appendix VID of the 2010 edition of the Chinese Pharmacopoeia, determination:

[0067] Chromatographic conditions and system adaptability test: octadecylsilane bonded silica gel is used as filler, acetonitrile-water=40:70 is used as mobile phase, and the detection wavelength is 250nm;

[0068] Preparation of reference substance solution: take formononetin reference substance, dissolve it in 80% methanol, and make a solution containing 0.03mg formononetin reference substance per 1 mL as a reference substance solution;

[0069] Preparation of the test solution: Take 4g of the contents under the item of difference in the dosage of this preparation, add 45mL of 80% methanol, and extract with reflux in a water bath for 50 minutes, filter, and evaporate the filtrate to dryness. Once, take the continued filtrate as the test solution;

[0070] Determination: Precisely draw 15μL each of the ref

[0071] Example 3 Determination of the content of Caulis spatholobi in Kangfuling Capsules: According to Appendix VID of the 2010 edition of the Chinese Pharmacopoeia, determination:

[0072] Chromatographic conditions and system adaptability test: octadecylsilane bonded silica gel is used as a filler, acetonitrile-water=40:80 is used as the mobile phase, and the detection wavelength is 260nm;

[0073] Preparation of reference substance solution: take formononetin reference substance, dissolve it in 80% methanol, and make a solution containing 0.03mg formononetin reference substance per 1 mL as a reference substance solution;

[0074] Preparation of the test solution: Take 3g of the contents under the difference in the amount of this preparation, add 40 mL of 80% methanol, and extract for 70 minutes under reflux in a water bath, filter, evaporate the filtrate, and add 10 mL of 80% methanol to dissolve the residue. Once, take the continued filtrate as the test solution;

[0075] Determinat