Peptide fragment 418-429 in highly conserved region of HCV envelope protein and application thereof

[0043] Example 1 A pharmaceutical composition containing HCV highly conserved region peptide 418-429

[0044] The pharmaceutical composition containing the peptide 418-429 of the highly conserved region of HCV includes the peptide 418-429 of the highly conserved region on the HCV envelope protein, the pharmaceutical carrier is water for injection, the pharmaceutical excipient lubricant dimethyl silicone oil, Crosslinking agent sodium alginate, binder ethyl cellulose, preservative paraben, pharmaceutically acceptable barbiturate.

[0045] The preparation method of the 1-6 type HCV pharmaceutical composition is:

[0046] a. Obtain 536 sequences covering HCV genotypes 1-6 from the NCBI website (see literature: Deng K. etal.. PloS one 10, 2015, e0138756) and compare them to obtain a highly conserved HCV envelope protein The peptide segment 418-429 in the region, and the peptide segment was synthesized in Shanghai Gil Biochemical Company to obtain the amino acid peptide segment;

[0047] b.

[0050] Example 2 A vaccine for preventing and treating hepatitis C

[0051] The preventive and therapeutic hepatitis C vaccine includes the peptide 418-429 of the highly conserved region of the envelope protein and the coupling protein obtained by coupling KLH, Freud's adjuvant, preservative thimerosal, inactivator formaldehyde, stabilizer lactose And phosphate buffer salt.

[0052] The preparation method of the preventive and therapeutic hepatitis C vaccine is:

[0053] ①Couple the peptide 418-429 of the highly conserved region of HCV envelope protein with KLH to obtain the coupling protein; the coupling process of the peptide and KLH is in accordance with the manufacturer's instructions of CellMosaic's KLH-peptide conjugation kits Operating

[0054] ②Introduce the coupling protein obtained in step ① into isolated chimpanzee liver cells to obtain recombinant chimpanzee liver cells;

[0055] ③Add the inactivating agent formaldehyde to the recombinant chimpanzee liver cells obtained in st

[0058] Example 3 Immunization of Balb / c mice

[0059] Download a total of 536 genotype HCV sequences of 1-6 genotypes through the NCBI (https: / / www.ncbi.nlm.nih.gov / ) website, through the website https: / / www.ebi.ac.uk / Tools / msa / Sequence analysis on clustalo / obtains the peptide 418-429 of the highly conserved region of the HCV envelope protein; at the same time, because 685-693 is located in the transmembrane region of the envelope protein and is not exposed, in theory, neutralizing antibodies will not be produced. Used as a negative control peptide in the experiment of the present invention.

[0060] The peptide 418-429 and the negative control peptide 685-693 were synthesized at Shanghai Jier Biochemical Company, with a purity of over 99%, and coupled with KLH as immune antigens.

[0061] The two peptide immunization antigens each immunize 6 Balb / c mice aged 8-10 weeks, and 6 unimmunized negative control mice are set up. The specific immunization process and an