Early stroke electrochemical quantitative detection test strip with hollow gold and silver nanospheres

[0030] In this example, the NSE and S100-β paired antibodies used were monoclonal antibodies prepared by monoclonal antibody technology. The principle of double-antibody sandwich detection of NSE and S100-β antigens is used to detect specimens. When the specimens to be tested contain NSE and S100-β antigens, the antigens will first interact with the hollow gold-silver bimetals coupled with NSE and S100-β antibodies on the binding pad. Binding, as the chromatography progresses, the conjugate moves forward to the NSE and S100-β antibody detection line, and the NSE and S100-β antigen will combine with the coating antibody again to form a double-antibody sandwich complex and gather at the detection line In addition, the unbound hollow gold-silver bimetal coupled with NSE and S100-β antibody will continue to move forward, and when it reaches the quality control line C, it will bind to the rabbit anti-mouse IgG antibody, so the hollow gold-silver bimetal will also appear at C gather.

[0051] In the preparation steps of immune hollow gold-silver nanospheres: the addition amount of EDC and NHS is 5 times the molar amount of hollow gold-silver bimetallic carboxylation degree, and other steps are the same as in Example 1.

[0053] In the preparation step of the conjugate pad, the sucrose content in the conjugate pad treatment solution used was 10 wt%, and the other steps were the same as in Example 1.

[0054] The method of using the detection card of the present invention is as follows.

[0055] 1. Add sample

[0056] Take out the test card for a single person from the packaging box, tear open the aluminum foil packaging bag, place it on a flat table, use a micropipette to pipette 70 μL of serum sample into the sample hole of the test card, and wait for the reaction to proceed for 20 minutes.