Method for producing fuel ethanol by fermentation of green alga biomass

[0026] A method for producing fuel ethanol by fermenting green algae biomass is as follows:

[0027] (1) Preparation of Chlorella Hydrolyzed Sugar Solution:

[0028] Step 1: after crushing Ulva and Enteromorpha respectively, weigh a certain amount of green algae powder to make 350g / L raw material.

[0029] Step 2: Add liquefaction enzyme (80IU / g) and glucoamylase (40IU / g) to the raw material in step 1 in sequence, the liquefaction enzyme treatment condition is 60°C, pH value is 6, the glucoamylase treatment condition is 60°C, pH value Under the condition of 4.5, they were hydrolyzed for 48 and 36 hours respectively.

[0030] Step 3: acid hydrolyze the raw material after enzymatic hydrolysis with 2% sulfuric acid, treat at 120°C for 2h, centrifuge at 3000r for 10min, and collect the supernatant.

[0031] Step 4: Adjust the pH of the hydrolyzed sugar solution to 5.5 with calcium hydroxide. Sterilize at 106°C for 30 minutes before use.

[0032] Step 5: Do three parallel fe

[0038] A method for producing fuel ethanol by fermenting green algae biomass is as follows:

[0039] (1) Preparation of Chlorella Hydrolyzed Sugar Solution:

[0040] Step 1: after crushing Ulva and Enteromorpha respectively, weigh a certain amount of green algae powder to make 350g / L raw material.

[0041] Step 2: Add liquefaction enzyme (80IU / g) and glucoamylase (40IU / g) to the raw material in step 1 in sequence, the liquefaction enzyme treatment condition is 60°C, pH value is 6, the glucoamylase treatment condition is 60°C, pH value Under the condition of 4.5, they were hydrolyzed for 48 and 36 hours respectively.

[0042] Step 3: acid hydrolyze the raw material after enzymolysis with 4% sulfuric acid, treat at 120°C for 2h, centrifuge at 3000r for 10min, and collect the supernatant.

[0043] Step 4: Adjust the pH of the hydrolyzed sugar solution to 5.5 with calcium hydroxide. Sterilize at 106°C for 30 minutes before use.

[0044] Step 5: Do three parallel fermentatio

[0050] A method for producing fuel ethanol by fermenting green algae biomass is as follows:

[0051] (1) Preparation of Chlorella Hydrolyzed Sugar Solution:

[0052] Step 1: after crushing Ulva and Enteromorpha respectively, weigh a certain amount of green algae powder to make 350g / L raw material.

[0053] Step 2: Add liquefaction enzyme (80IU / g) and glucoamylase (40IU / g) to the raw material in step 1 in sequence, the liquefaction enzyme treatment condition is 60°C, pH value is 6, the glucoamylase treatment condition is 60°C, pH value Under the condition of 4.5, they were hydrolyzed for 48 and 36 hours respectively.

[0054] Step 3: acid hydrolyze the raw material after enzymolysis with 8% sulfuric acid, treat at 120° C. for 2 hours, centrifuge at 3000 r for 10 minutes, and collect the supernatant.

[0055] Step 4: Adjust the pH of the hydrolyzed sugar solution to 5.5 with calcium hydroxide. Sterilize at 106°C for 30 minutes before use.

[0056] Step 5: Do three paralle