Research method for increasing oxidation resistance of Ilex verticillata

A technology of anti-oxidation ability and holly, applied in the field of North American holly growth research, can solve the problems of limited popularization and application, and low drought resistance of North American holly

Inactive Publication Date: 2020-02-21
ZHEJIANG FORESTRY UNIVERSITY
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AI Technical Summary

Benefits of technology

This patented technology allows us to measure certain physical or chemical properties that are associated with plants called snowy weather-resistant genes found within these species. By comparing them against each other we could determine which ones have better ability to resist damage from waterlogging compared to others. Additionally, this analysis helps predict how well it will be during severe conditions like droughts without causing harmful consequences such as reduced yielding rates due to poor nutritional content.

Problems solved by technology

This patents discuss how manganese ion plays a crucial part during saltwater stressed conditions when crops cannot produce enough salts due to insufficient nutrients or other factors like high temperatures. It suggests finding ways to enhance this ability without causing damage from these harsh environments.

Method used

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  • Research method for increasing oxidation resistance of Ilex verticillata
  • Research method for increasing oxidation resistance of Ilex verticillata
  • Research method for increasing oxidation resistance of Ilex verticillata

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Embodiment Construction

[0026] A kind of research method of the present invention improves North American Ilex antioxidant capacity, comprises the following steps:

[0027] Step 1: Experimental materials and processing

[0028] The test materials were two-year-old cuttings of two North American holly varieties Ilex verticillata 'Oosterwijk' (Oosterwijk) and Ilex verticillata 'Apollo' (Apollo) with the same growth. In late March, all the seedlings were transplanted into plastic pots of the same specification with a diameter of 16 cm and a depth of 15 cm, and one plant was planted in each pot. Each pot contains 1.7kg of cultivation soil, which is made by mixing garden soil and nutrient matrix at a ratio of 3:1. After transplanting, the seedlings are slowly seeded in the greenhouse for 2 months, and 80%-85% of the normal soil field water holding capacity is maintained during the growth process for daily watering without fertilization.

[0029] In mid-June of that year, 18 cutting seedlings of two varieti

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Abstract

The invention provides a research method for increasing the oxidation resistance of Ilex verticillata. The method includes: selecting the biennial cuttage seedlings of two Ilex verticillata varieties,and performing acclimation in a greenhouse for 2 months; selecting 18 cuttage seedlings of each of the two varieties, separating each variety into two groups, spraying melatonin to one group, and spraying equal-amount clean water to the other group; performing identical drought stress treatment at the same time; collecting fresh leaves immediately after the treatment, on the seventh day after thetreatment and on the thirtieth day after the treatment, quickly freezing the collected leaves in liquid nitrogen, and preserving the frozen leaves in refrigerator of ultralow temperature of minus 80DEG C; measuring superoxide dismutase and peroxidase activity and malondialdehyde content. The research method has the advantages that by applying external melatonin to perform treatment, the influence of the melatonin on the oxidation resistance of the Ilex verticillata cuttage seedlings under drought stress is comparatively analyzed; the research results can lay a foundation for the research ofthe Ilex verticillata physiological mechanism responding to the drought stress and measures for increasing the drought resistance of the Ilex verticillata and the popularization of the Ilex verticillata in drought regions.

Description

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Claims

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Application Information

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Owner ZHEJIANG FORESTRY UNIVERSITY
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