Culture medium for promoting regeneration of gourd cotyledons and application of culture medium

[0027] Example 1

[0028] A method for promoting the regeneration of cucurbit cotyledons, comprising the steps:

[0029] 1) Aseptic seedling cultivation: select 30 gourd seeds with full particles and the same size, peel off the outer seed coat by hand, and leave the seed kernels. times), inoculated into 0.6% agar medium, inoculated at a density of 10 seed kernels / Petri dish, and cultivated for 3 days under dark conditions to obtain aseptic seedlings of cucurbits. At this time, the gourd cotyledons are goose yellow and have not yet unfolded;

[0030] 2) According to figure 1 As shown, cut two cotyledons of the gourd to obtain 8 cotyledon explants / sterile seedlings, totaling 240 cotyledon explants; the cutting method is specifically:

[0031] 21) fix the radicle of the aseptic seedlings of the gourd, and cut off the top leaf margin of the cotyledons;

[0032] 22) on the basis of step 1), cut off the leaf margins on both sides of the cotyledons;

[0033] 23) on the basis of