Bioink composition for cartilage regeneration, method for manufacturing customized scaffold for cartilage regeneration using same, and customized scaffold for cartilage regeneration manufactured using manufacturing method

FOR CONFIRMATION OF CHONDROGENIC DIFFERENTIATION (IN VITRO EXPERIMENT)

[0069]FIG. 1 illustrates a process of manufacturing a pellet for confirming cartilage differentiation ability. Specifically, an experiment for confirming cartilage differentiation ability was performed as follows.

[0070]1. The obtained SVF (105 to 107 cells) was mixed with physiological saline in which 10 mg (Example 1), 50 mg (Example 2), and 100 mg (Example 3) of the prepared hyaline cartilage powder (MCCM) were dispersed.

[0071]2. After this mixed liquid was centrifuged at 1000 rpm at 4° C. for 5 minutes, an MCCM / SVF mixture was obtained by removing the physiological saline supernatant.

[0072]3. The MCCM / SVF mixture was mixed with 1 ml of an aprotinin liquid in which fibrinogen was mixed using a mix syringe,

[0073]4. 1 ml of the MCCM / SVF / fibrinogen solution and 1 ml of a calcium chloride solution in which thrombin was dispersed were each connected to a Y piece, and 20 μl of each solution was dispensed into a 15-ml conical tube and hardened at room temperature for 5 minutes to form a ...

[0077]FIG. 6 illustrates an animal experimental procedure for confirming whether the cartilage is regenerated. Specifically, an animal experiment for confirming whether the cartilage was regenerated was performed as follows.

[0078]1. The obtained SVF (105 to 107 cells) were mixed with physiological saline in which 10 mg of the prepared hyaline cartilage powder (MCCM) was dispersed.

[0079]2. After this mixed liquid was centrifuged at 1000 rpm at 4° C. for 5 minutes, an MCCM / SVF mixture was obtained by removing the physiological saline supernatant.

[0080]3. The MCCM / SVF mixture was mixed using 1 ml of an aprotinin solution in which fibrinogen was mixed and a mix syringe.

[0081]4. 1 ml of the MCCM / SVF / fibrinogen solution and 1 ml of a calcium chloride solution in which thrombin was dispersed were each prepared.

[0082]5. A knee femoral condyle of an experimental beagle was exposed, and a defect region having a diameter of about 6 mm and a depth of about 2 mm was formed in this part.

[0083]6. ...