Bacillus coagulans and method for catalytically producing 2'-deoxyadenosine by bacillus coagulans

[0054] Embodiment 1: strain source

[0055] Bacillus coagulans (Bacillus coagulans) DHE002 (CGMCC NO.23118) was isolated from the soil on the slope of Mount Tiantai, Taizhou City, Zhejiang Province, China.

[0056] Cross-sampled the soil in the Tiantai Mountain area, randomly selected 5 sampling points, took 10g of soil samples from each point, put them into a conical flask, mixed them evenly, took 10g of samples, and added them to a conical flask filled with 90mL of sterile water. In the bottle (there is a magnetic stirrer in the bottle), vortex and stir for 30 minutes, make it fully mixed to make a suspension, which is 10 -1 bacterial suspension. Dilute the above suspension with sterile water at a volume ratio of 1:9 to 10 by dilution coating plate method. -2 , 10 -3 , 10 -4 , 10 -5 Concentration, take 0.1mL of bacterial suspension with different dilution multiples, spread it on the beef extract peptone agar medium plate, gently spread it on the surface of the medium with

[0060] Example 2: Morphology, cultural characteristics, physiological and biochemical characteristics of Bacillus coagulans (Bacillus coagulans) DHE002 (CGMCC NO.23118).

[0061] The colony characteristics of strain DHE002 cultured in beef extract peptone solid medium at 30°C for 24 hours are as follows: figure 1 As shown, the colony is flat and milky white, raised, opaque, indicating dryness, and the color of the front and back is the same. Gram staining of bacteria was positive, rod-shaped under microscope, and spores existed in some bacteria.

[0062] Refer to the relevant content in "Bergey's Handbook of Systematic Bacteriology", "Handbook of Common Bacterial System Identification" and other books for identification. The physiological responses of the strains are shown in Table 1.

[0063] Table 1. Physiological response of Bacillus coagulans (Bacillus coagulans) DHE002 (CGMCC NO.23118)

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[0065]

[0066] Remarks: +: Positive; -: Negative.

[0067] Embodiment 3 strain identification

[0068] The 16S rDNA sequence analysis of Bacillus coagulans (Bacillus coagulans) DHE002 (CGMCC NO.23118) was carried out according to the relevant content in the book "Molecular Cloning Experiment Guide". The bacteria were collected, and then the total DNA was extracted with a bacterial DNA extraction kit.

[0069] Design primers: Forward primer27F (5'-AGAGTTTGATCCTGGCTCAG-3'), Revese primer1492R (5'-GGTTACCTTGTTACGACTT-3', for PCR amplification, PCR product detection using 0.8% agarose gel electrophoresis, PCR product purification and recovery using SanPrep column Formula PCR purification product kit, the purified PCR product was directly sent to Sangon Bioengineering (Shanghai) Co., Ltd. for sequence determination.

[0070] After proofreading, the 16S rDNA sequence of strain DHE002 (CGMCC NO.23118) was checked and compared with the sequences of related species and genus in the GenBank database by homologous sequence BLAST to determin