Biosynthesis method from formic acid to formaldehyde and/or methanol

[0044] Embodiment 1 The new way of synthesizing formaldehyde from formic acid

[0045] A new pathway for the synthesis of formaldehyde from formic acid is shown below:

[0046]

[0047] First, catalysts (ie, enzymes) that can catalyze each chemical reaction in the pathway are selected (see Table 1), but are not limited to the catalysts listed in Table 1. Then, different catalysts were combined to establish a corresponding reaction system, and after a period of reaction, the output of formaldehyde was detected.

[0048] The information of enzyme used in embodiment 1 is listed in table 1, and acetate kinase, phosphate acetyltransferase, acetaldehyde dehydrogenase, formate dehydrogenase are obtained by PCR or the mode of gene synthesis, and by Simple Cloning (You, C., et al.(2012)."Simple Cloning via Direct Transformation of PCR Product(DNAMultimer)to Escherichia coli and Bacillus subtilis."Appl.Environ.Microbiol.78(5):1593-1595.) method of cloning into pET20b, pET21b and pET28

[0057] Embodiment 2 The new way of synthesizing methanol from formic acid

[0058] A new pathway for the synthesis of methanol from formic acid is shown below:

[0059]

[0060] First, a catalyst is selected that can catalyze each chemical reaction in the pathway (see Table 2), but is not limited to the catalysts listed in Table 2. Then, different catalysts were combined to establish a corresponding reaction system, and after a period of reaction, the output of methanol was detected.

[0061] The information of the enzymes used in Example 2 is listed in Table 2. Alcohol dehydrogenase was purchased from Sigma (https: / / www.sigmaaldrich.com / china-mainland.html). The construction and expression methods of other enzymes are the same as in Example 1.

[0062] Table 2 Catalysts used for the new pathway chemical reaction from formic acid to methanol

[0063]

[0064] Methanol production was detected by the following method: using Agilent 7890A chromatograph (including FID