Gene recombination human interferon alpha 2b liposome and injection, suppository, eye drop, sparying preparation and ointment thereof

[0019] Example 1, recombinant human interferon α2b liposome

[0020] prescription:

[0021] Soybean lecithin for injection 125mg, cholesterol 15.25mg, fat oil 75μg, colloidal silicon 40μg, recombinant human interferon α2b 6×10 6 IU.

[0022] Preparation method: Weigh soybean lecithin and cholesterol for injection, put them in a round bottom flask, add chloroform:ethanol as 1:1 mixed solvent appropriate amount, dissolve, evaporate under reduced pressure on a rotary evaporator to remove the solvent, and continue vacuum drying for 12 hours , form a lipid film on the wall of the flask, add fatty oil and colloidal silica gel (containing medicine), hydrate in a water bath at 40°C for 1 hour, stir magnetically for 1 hour, cool to room temperature, and obtain. Under sterile conditions, filter through a microporous membrane of 0.45 μg, separate the free drug by high-speed centrifugation (250000r / m, 30min), take the clear liquid and use the CPE method to measure the biological activity

[0023] Example 2, recombinant human interferon α2b liposome

[0024] prescription:

[0025] Soybean lecithin for injection 150mg, cholesterol 50mg, fatty oil 200μg, colloidal silicon 200μg, recombinant human interferon 8×10 7 IU.

[0026] The method of making is the same as above.

[0027] Example 3, Gene Recombinant Human Interferon α2b Liposome

[0028] prescription:

[0029] Soybean lecithin for injection 45mg, cholesterol 5mg, fatty oil 10μg, colloidal silicon 10μg, recombinant human interferon 2×10 6 IU.

[0030] The method of making is the same as above.