Method and apparatus for cell recovery

Inactive Publication Date: 2005-02-03
HITACHI LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0026] In accordance with the method and apparatus of the invention for cell recovery, good, living cancer cells can be recovered from stool at room temperature, in contrast to the cooling method as disclosed in JP Patent Publication (Kohyo) No. 11-511982 A (1999) (WO97/09600), whereby the surface of a cooled and frozen stool is scraped and then cancer cells existing in the surface of the stool are exfoliated. Thus, in accordance with the invention, the recovered cells can be subjected to cytological, immunological and biochemical analyses with high accuracy. Also, the method and apparatus of the inventi

Problems solved by technology

However, in the existing method whereby cells are recovered from the surface of stool that is frozen by cooling, as described in JP Patent Publication (Kohyo) No. 11-511982 A (1999) (WO97/09600) (to be hereafter referred to as a cooling method), the operation is bothersome and the screening cannot be performed on a large scale.
In the cooling method, there is the step of centrifugation, which takes time and does not render itself to automation and

Method used

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examples

[0067] Hereafter, an example of the invention will be described. The invention, however, is not limited to the example.

[0068] (Recovery of Cells from Stool)

[0069] Stool voided by a colorectal cancer patient prior to operation was used as a specimen. As to the use of stool, the subject was informed of the content of the procedure prior to the experiment and a written consent was obtained.

[0070] Two hundred mL of Hanks solution (Nissui) containing 10% FBS was put into a stomacher bag containing stool (about 5 to 80 g) and the bag was then sealed. A stool suspension was then prepared by using a stomacher (200 rpm, 1 min).

[0071] In the case where a stomacher bag having a filter was used, the suspension was filtered by the filter in the bag. In the case where a stomacher bag without a filter was used, the suspension was filtered by passing it through a funnel-shaped filter set on a tubular plastic container. The filtered solution was recovered in a beaker. The filtered solution was furt

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Abstract

A method and apparatus for recovering cells from stool are provided for diagnosing colorectal cancer from stool naturally voided by multiple specimens. The method includes the steps of preparing a sample of naturally voided and collected stool, to which sample a buffer solution is added, causing cancer cells in the sample from which the impurities have been removed to be adsorbed on a solid carrier, and recovering the cancer cells thus adsorbed.

Description

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Claims

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Application Information

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Owner HITACHI LTD
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