Plasma miRNA markers related to auxiliary diagnosis of colorectal cancer and application of the markers

[0036] From 2013 to 2015, the inventor collected a large number of venous plasma samples from patients with colorectal cancer and normal physical examination population from the First Affiliated Hospital of Nanjing Medical University, and selected 139 cases of colorectal cancer and 132 cases of The normal control sample was used as the experimental sample for the initial screening of Exiqon miRNA qPCR panel chip and subsequent series of qRT-PCR verification. At the same time, 24 cases of colorectal cancer tissues and 24 cases of paracancerous tissues were retained. The selected patients' plasma samples were all from newly diagnosed patients who had not undergone surgery, radiotherapy and chemotherapy intervention and were pathologically confirmed as colorectal cancer. The demographic data and clinical data of these samples were collected systematically.

[0037] Refer to the flowchart ( figure 1 ), randomly selected 30 colorectal cancer samples and 10 normal controls from the p