Use of p2x7 pathway for assessing the sensitivity of a subject to a cancer treatment

[0173]Material and Methods

[0174]Mouse Strains

[0175]BALB / c (H-2d), C57BL / 6 (H-2b), nu / nu and Rag2− / − C57BL / 6 mice were obtained from the Centre d'élevage Janvier (Le Genest St Isle, France) and from Charles River Laboratories (L'Arbresle, France), as well as from Taconic (Denmark).

[0176]C57BL / 6 Ifnγr1− / −, Casp1− / −, Asc− / −, Nalp3− / −, P2X7− / −and IL-12Rβ2− / − mice were described in Huang et al, 1993; Li et al., 1995; Srinivasula et al., 2002; Kanneganti et al., 2006; Solle et al., 2001; and Wu et al., 2000, respectively. Mice were bred in pathogen-free conditions. Animals were used between 6 and 20 weeks of age. All animals were maintained according to both the FELASA guidelines and the Animal Experimental Ethics Committee Guidelines (Val de Marne, France).

[0177]Reagents and Materials

[0178]Cell death was induced either with doxorubicin, mitoxantrone (Sigma Aldrich, St Quentin Fallavier, France), or oxaliplatin (Sanofi-Aventis, France). Ovalbumin protein was purchased