Hippocampus-associated proteins, DNA sequences coding therefor and uses thereof

a technology of hippocampus and dna sequences, applied in the field of hippocampus-associated proteins, can solve the problems of inability to learn a spatial navigation task, inability to fully understand the biological role of steroid metabolizing cyp's in vivo, and inability to fully understand the full spectrum of steroid metabolizing cyp's in the brain, so as to improve cognitive function and reduce hepatic expression.

Inactive Publication Date: 2001-02-06
BTG INT LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

The patented method allows for detection of specific proteins called histones involved in regulating necrosis or apoptosis pathways during development processes such as embryonic deaths from injury or cancer treatment. This can help identify those cells affected by these treatments more accurately than current methods like immunohistochemistry which require tissue culture techniques.

Problems solved by technology

This patent describes various technical problem addressed in this patented patent relational texts about how certain types of DNA can form complex structures called chromoplexes when subjected to specific binding interactions between them. Existing methods involve extracting these chromophores through techniques like electrosplicidification procedures or cloning origns into smaller pieces followed by amplifying their respective genome segments. However, existing methods have limitations including lack sensitivity due either to low efficiency or high cost associated with current technologies.

Method used

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  • Hippocampus-associated proteins, DNA sequences coding therefor and uses thereof
  • Hippocampus-associated proteins, DNA sequences coding therefor and uses thereof
  • Hippocampus-associated proteins, DNA sequences coding therefor and uses thereof

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Embodiment Construction

Details of the isolation of hippocampus-associated DNA molecules according to the invention will now be described by way of example:

1. ISOLATION OF GENE ENCODING RAT HCT-1

1.1 Differential screening of a rat hippocampus cDNA library

To identify genes whose expression is enriched in the hippocampal formation we performed a differential hybridization screen of a hippocampal cDNA library. Adult rat hippocampal RNA was reverse transcribed using a oligo-dT-NotI primer, converted to double-stranded cDNA, EcoRI adaptors were attached and the cDNA's were inserted between the EcoRI and NotI sites of a bacteriophage lamda vector.

1.1.1 Preparation of cDNA libraries

Following anaesthesia (sodium pentobarbital) of adult rats (Lister hooded) the hippocampal formation was dissected, including areas CA1-3 and dentate gyrus, subiculum, alvear and fimbrial fibres but excluding fornix and afferent structures such as septum and entorhinal cortex. Remainder of brain was also pooled taking care to exclude hipp

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Abstract

This invention provides novel hippocampus-associated proteins and DNA sequences coding therefor. In an investigation of hippocampus-associated proteins by differential screening of a rat hippocampus cDNA library, a cDNA species encoding a novel protein designated Hct-1 was isolated and shown to be a to cytochromes P450. The use of hybridization probes based on the rat Hct-1 sequence has led to the identification of homologues in other mammalian species.

Description

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Claims

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Application Information

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Owner BTG INT LTD
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